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Dhcr24 (NM_053272) Mouse Tagged ORF Clone
CAT＃: MG208301
- TrueORF®
Dhcr24 (tGFP-tagged) - Mouse 24-dehydrocholesterol reductase (Dhcr24)
ORF Plasmid: DDK
"NM_053272" in other vectors (4)
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CNY 4,940.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2310076D10Rik; 5830417J06Rik; mKIAA0018 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG208301 representing NM_053272
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCCGCCGTGTCGCTGGCCGTGTGCGCGCTGCTCTTTCTGCTCTGGGTGCGAGTGAAGGGGTTGG AGTTCGTTCTCATCCACCAGCGCTGGGTGTTCGTGTGCCTCTTCTTGCTGCCGCTCTCGCTCATCTTCGA TATCTACTACTACGTGCGCGCCTGGGTGGTGTTCAAGCTGAGCAGTGCGCCGCGCCTGCACGAGCAGCGC GTGCGGGACATCCAGAAACAGGTCCGGGAATGGAAGGAACAGGGCAGTAAGACCTTCATGTGCACGGGGC GCCCAGGCTGGCTCACTGTCTCGCTGCGAGTCGGAAAGTACAAGAAGACCCATAAGAACATCATGATCAA CCTGATGGACATCCTGGAGGTGGACACCAAGAAACAGATTGTTCGAGTGGAGCCCTTGGTGTCTATGGGT CAGGTGACAGCTTTGCTGAACTCCATTGGCTGGACCCTGCCTGTGTTGCCTGAGCTTGATGACCTCACAG TGGGGGGCCTGATCATGGGCACAGGCATCGAGTCATCGTCCCACAAGTATGGCCTGTTCCAACACATTTG CACTGCCTACGAGCTGATCCTGGCAGACGGCAGCTTTGTGCGCTGCACACCGTCTGAAAACTCAGACCTG TTCTATGCCGTGCCCTGGTCCTGTGGGACCCTGGGCTTCCTGGTGGCTGCCGAGATCCGGATCATCCCGG CCAAGAAGTATGTCAAGCTGCGGTTTGAGCCTGTTCGGGGCCTGGAGGCCATCTGTGAAAAATTCACCCG CGAGTCCCAGCGGCTGGAGAACCACTTCGTGGAAGGGTTGCTGTACTCCCTGGATGAGGCTGTCATCATG ACAGGGGTCATGACGGACGACGTAGAGCCCAGCAAGCTGAATAGCATTGGCAGTTACTACAAGCCCTGGT TCTTCAAGCATGTGGAGAACTACCTGAAGACAAACCGGGAGGGCCTCGAATACATTCCCCTGAGACACTA CTACCACCGACACACGCGCAGCATCTTCTGGGAGCTCCAGGACATCATCCCTTTCGGCAACAACCCCATC TTCCGCTACCTCTTCGGCTGGATGGTGCCTCCCAAGATCTCCCTCCTGAAGCTGACCCAGGGCGAGACGC TACGCAAGCTGTACGAGCAGCACCACGTGGTGCAGGACATGCTGGTGCCCATGAAGTGCATGTCACAGGC CCTGCATACCTTCCAAAATGACATCCACGTCTACCCCATCTGGCTGTGCCCATTCATCCTGCCCAGCCAG CCAGGACTAGTGCATCCCAAGGGAGATGAAGCAGAGCTCTACGTGGACATCGGGGCATACGGGGAGCCAC GTGTGAAGCACTTCGAGGCCAGGTCCTGCATGAGGCAGCTGGAGAAGTTTGTGCGGAGTGTGCACGGGTT CCAAATGTTATACGCCGATTGCTATATGAACCGCGAGGAATTCTGGGAGATGTTCGATGGCTCCTTGTAC CACAAGCTGCGCAAGCAGCTGGGCTGCCAGGACGCCTTCCCTGAGGTGTACGACAAGATCTGCAAGGCGG CAAGGCAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208301 representing NM_053272
Red=Cloning site Green=Tags(s) MEPAVSLAVCALLFLLWVRVKGLEFVLIHQRWVFVCLFLLPLSLIFDIYYYVRAWVVFKLSSAPRLHEQR VRDIQKQVREWKEQGSKTFMCTGRPGWLTVSLRVGKYKKTHKNIMINLMDILEVDTKKQIVRVEPLVSMG QVTALLNSIGWTLPVLPELDDLTVGGLIMGTGIESSSHKYGLFQHICTAYELILADGSFVRCTPSENSDL FYAVPWSCGTLGFLVAAEIRIIPAKKYVKLRFEPVRGLEAICEKFTRESQRLENHFVEGLLYSLDEAVIM TGVMTDDVEPSKLNSIGSYYKPWFFKHVENYLKTNREGLEYIPLRHYYHRHTRSIFWELQDIIPFGNNPI FRYLFGWMVPPKISLLKLTQGETLRKLYEQHHVVQDMLVPMKCMSQALHTFQNDIHVYPIWLCPFILPSQ PGLVHPKGDEAELYVDIGAYGEPRVKHFEARSCMRQLEKFVRSVHGFQMLYADCYMNREEFWEMFDGSLY HKLRKQLGCQDAFPEVYDKICKAARH TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_053272 |
| ORF Size | 1548 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_053272.2, NP_444502.2 |
| RefSeq Size | 4028 bp |
| RefSeq ORF | 1551 bp |
| Locus ID | 74754 |
| UniProt ID | Q8VCH6 |
| Gene Summary | Catalyzes the reduction of the Delta-24 double bond of sterol intermediates.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203901 | Dhcr24 (untagged) - Mouse 24-dehydrocholesterol reductase (Dhcr24), (10ug) |
CNY 2,000.00 |
|
| MR208301 | Dhcr24 (Myc-DDK-tagged) - Mouse 24-dehydrocholesterol reductase (Dhcr24) |
CNY 3,848.00 |
|
| MR208301L3 | Lenti ORF clone of Dhcr24 (Myc-DDK-tagged) - Mouse 24-dehydrocholesterol reductase (Dhcr24) |
CNY 6,370.00 |
|
| MR208301L4 | Lenti ORF clone of Dhcr24 (mGFP-tagged) - Mouse 24-dehydrocholesterol reductase (Dhcr24) |
CNY 6,370.00 |
