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Acaa2 (NM_177470) Mouse Tagged ORF Clone
CAT＃: MG206222
- TrueORF®
Acaa2 (tGFP-tagged) - Mouse acetyl-Coenzyme A acyltransferase 2 (mitochondrial 3-oxoacyl-Coenzyme A thiolase) (Acaa2)
ORF Plasmid: DDK
"NM_177470" in other vectors (4)
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CNY 3,710.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 0610011L04Rik; AI255831; AI265397; D18Ertd240e |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG206222 representing NM_177470
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCTGCTACGAGGTGTGTTCATCGTCGCTGCGAAGCGAACACCCTTTGGAGCTTACGGGGGCCTTC TCAAGGACTTCTCTGCCACCGATTTAACTGAATTTGCTGCCAGGGCTGCTCTGTCTGCTGGCAAAGTTCC ACCTGAAACCATCGATAGTGTCATCGTGGGCAATGTCATGCAGAGCTCTTCAGATGCGGCATACCTGGCG AGGCATGTGGGTTTGCGAGTGGGAGTCCCAACAGAGACTGGGGCACTTACCCTCAACAGGCTCTGTGGCT CTGGTTTCCAGTCCATCGTGAGCGGATGTCAGGAAATCTGTTCTAAAGATGCTGAGGTCGTCTTGTGTGG AGGAACAGAGAGCATGAGCCAGTCCCCCTACTGTGTCAGAAATGTGCGCTTCGGAACCAAATTTGGATTA GATCTCAAGCTGGAAGATACTTTGTGGGCAGGATTAACGGATCAACATGTTAAGCTGCCCATGGGAATGA CTGCAGAGAACCTTGCTGCAAAATACAACATAAGCAGAGAAGACTGTGACAGATACGCCTTGCAGTCTCA GCAGAGGTGGAAAGCTGCTAACGAGGCTGGCTACTTCAATGAGGAGATGGCACCCATTGAGGTGAAGACG AAGAAAGGCAAACAGACCATGCAAGTGGACGAGCACGCTCGACCCCAAACCACCCTGGAGCAACTGCAGA AGCTCCCGTCCGTGTTCAAGAAAGACGGGACAGTCACAGCAGGGAACGCCTCGGGGGTGTCTGACGGTGC TGGGGCCGTCATCATAGCCAGCGAAGATGCTGTCAAAAAACATAACTTCACGCCCCTGGCCAGAGTCGTG GGCTACTTCGTGTCCGGATGCGATCCTACTATCATGGGTATTGGTCCAGTCCCTGCTATCAATGGAGCAT TGAAGAAAGCTGGGCTGAGTCTTAAGGACATGGATTTGATAGACGTGAACGAAGCTTTTGCCCCTCAGTT CTTGTCTGTTCAGAAGGCCCTGGATCTTGACCCCAGCAAAACCAATGTGAGTGGAGGCGCCATTGCCCTG GGTCACCCGCTGGGAGGATCTGGCTCCAGAATCACCGCACACCTGGTTCATGAGTTAAGGCGTCGAGGTG GAAAGTACGCAGTGGGATCGGCTTGCATTGGAGGTGGCCAAGGCATCGCCCTGATCATCCAGAACACAGT C ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG206222 representing NM_177470
Red=Cloning site Green=Tags(s) MALLRGVFIVAAKRTPFGAYGGLLKDFSATDLTEFAARAALSAGKVPPETIDSVIVGNVMQSSSDAAYLA RHVGLRVGVPTETGALTLNRLCGSGFQSIVSGCQEICSKDAEVVLCGGTESMSQSPYCVRNVRFGTKFGL DLKLEDTLWAGLTDQHVKLPMGMTAENLAAKYNISREDCDRYALQSQQRWKAANEAGYFNEEMAPIEVKT KKGKQTMQVDEHARPQTTLEQLQKLPSVFKKDGTVTAGNASGVSDGAGAVIIASEDAVKKHNFTPLARVV GYFVSGCDPTIMGIGPVPAINGALKKAGLSLKDMDLIDVNEAFAPQFLSVQKALDLDPSKTNVSGGAIAL GHPLGGSGSRITAHLVHELRRRGGKYAVGSACIGGGQGIALIIQNTV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_177470 |
| ORF Size | 1191 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_177470.2, NP_803421.1 |
| RefSeq Size | 1502 bp |
| RefSeq ORF | 1194 bp |
| Locus ID | 52538 |
| UniProt ID | Q8BWT1 |
| Gene Summary | In the production of energy from fats, this is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA. Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain unbranched 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms. Also catalyzes the condensation of two acetyl-CoA molecules into acetoacetyl-CoA and could be involved in the production of ketone bodies. Also displays hydrolase activity on various fatty acyl-CoAs (By similarity). Thereby, could be responsible for the production of acetate in a side reaction to beta-oxidation (By similarity). Abolishes BNIP3-mediated apoptosis and mitochondrial damage (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
H2A.Z-dependent and-independent recruitment of metabolic enzymes to chromatin required for histone modifications
,Choi, S;Jeon, YH;Yang, Z;He, M;Shin, H;Pfleger, J;,
bioRxiv
[ACAA2]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203155 | Acaa2 (untagged) - Mouse acetyl-Coenzyme A acyltransferase 2 (mitochondrial 3-oxoacyl-Coenzyme A thiolase) (Acaa2), nuclear gene encoding mitochondrial protein, (10ug) |
CNY 2,000.00 |
|
| MR206222 | Acaa2 (Myc-DDK-tagged) - Mouse acetyl-Coenzyme A acyltransferase 2 (mitochondrial 3-oxoacyl-Coenzyme A thiolase) (Acaa2), nuclear gene encoding mitochondrial protein |
CNY 3,330.00 |
|
| MR206222L3 | Lenti ORF clone of Acaa2 (Myc-DDK-tagged) - Mouse acetyl-Coenzyme A acyltransferase 2 (mitochondrial 3-oxoacyl-Coenzyme A thiolase) (Acaa2), nuclear gene encoding mitochondrial protein |
CNY 5,230.00 |
|
| MR206222L4 | Lenti ORF clone of Acaa2 (mGFP-tagged) - Mouse acetyl-Coenzyme A acyltransferase 2 (mitochondrial 3-oxoacyl-Coenzyme A thiolase) (Acaa2), nuclear gene encoding mitochondrial protein |
CNY 5,230.00 |
