Gna15 (NM_010304) Mouse Tagged ORF Clone

CAT#: MG205807

  • TrueORF®

Gna15 (tGFP-tagged) - Mouse guanine nucleotide binding protein, alpha 15 (Gna15)

ORF Plasmid: DDK tGFP



  "NM_010304" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 3,710.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms Galpha15; G[a]15
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG205807 representing NM_010304
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCGGTCCCTGACTTGGGGCTGCTGTCCCTGGTGCCTGACAGAGGAGGAGAAGACTGCCGCCAGAA
TCGACCAGGAGATCAACAGGATTTTGTTGGAACAGAAAAAACAAGAGCGCGAGGAATTGAAACTCCTGCT
GTTGGGGCCTGGTGAGAGCGGGAAGAGTACGTTCATCAAGCAGATGCGCATCATTCACGGTGTGGGCTAC
TCGGAGGAGGACCGCAGAGCCTTCCGGCTGCTCATCTACCAGAACATCTTCGTCTCCATGCAGGCCATGA
TAGATGCGATGGACCGGCTGCAGATCCCCTTCAGCAGGCCTGACAGCAAGCAGCACGCCAGCCTAGTGAT
GACCCAGGACCCCTATAAAGTGAGCACATTCGAGAAGCCATATGCAGTGGCCATGCAGTACCTGTGGCGG
GACGCGGGCATCCGTGCATGCTACGAGCGAAGGCGTGAATTCCACCTTCTGGACTCCGCGGTGTATTACC
TGTCACACCTGGAGCGCATATCAGAGGACAGCTACATCCCCACTGCGCAAGACGTGCTGCGCAGTCGCAT
GCCCACCACAGGCATCAATGAGTACTGCTTCTCCGTGAAGAAAACCAAACTGCGCATCGTGGATGTTGGT
GGCCAGAGGTCAGAGCGTAGGAAATGGATTCACTGTTTCGAGAACGTGATTGCCCTCATCTACCTGGCCT
CCCTGAGCGAGTATGACCAGTGCCTAGAGGAGAACGATCAGGAGAACCGCATGGAGGAGAGTCTCGCTCT
GTTCAGCACGATCCTAGAGCTGCCCTGGTTCAAGAGCACCTCGGTCATCCTCTTCCTCAACAAGACGGAC
ATCCTGGAAGATAAGATTCACACCTCCCACCTGGCCACATACTTCCCCAGCTTCCAGGGACCCCGGCGAG
ACGCAGAGGCCGCCAAGAGCTTCATCTTGGACATGTATGCGCGCGTGTACGCGAGCTGCGCAGAGCCCCA
GGACGGTGGCAGGAAAGGCTCCCGCGCGCGCCGCTTCTTCGCACACTTCACCTGTGCCACGGACACGCAA
AGCGTCCGCAGCGTGTTCAAGGACGTGCGGGACTCGGTGCTGGCCCGGTACCTGGACGAGATCAACCTGC
TG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG205807 representing NM_010304
Red=Cloning site Green=Tags(s)

MARSLTWGCCPWCLTEEEKTAARIDQEINRILLEQKKQEREELKLLLLGPGESGKSTFIKQMRIIHGVGY
SEEDRRAFRLLIYQNIFVSMQAMIDAMDRLQIPFSRPDSKQHASLVMTQDPYKVSTFEKPYAVAMQYLWR
DAGIRACYERRREFHLLDSAVYYLSHLERISEDSYIPTAQDVLRSRMPTTGINEYCFSVKKTKLRIVDVG
GQRSERRKWIHCFENVIALIYLASLSEYDQCLEENDQENRMEESLALFSTILELPWFKSTSVILFLNKTD
ILEDKIHTSHLATYFPSFQGPRRDAEAAKSFILDMYARVYASCAEPQDGGRKGSRARRFFAHFTCATDTQ
SVRSVFKDVRDSVLARYLDEINLL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_010304
ORF Size 1122 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_010304.3, NP_034434.1
RefSeq Size 1974 bp
RefSeq ORF 1125 bp
Locus ID 14676
UniProt ID P30678
Gene Summary Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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