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Prkacb (NM_011100) Mouse Tagged ORF Clone
CAT＃: MG205329
- TrueORF®
Prkacb (tGFP-tagged) - Mouse protein kinase, cAMP dependent, catalytic, beta (Prkacb)
ORF Plasmid: DDK
"NM_011100" in other vectors (6)
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CNY 7,456.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CbPKA; Pkacb |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG205329 representing NM_011100
Red=Cloning site Blue=ORF Green=Tags(s) CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC GCCC ATGGGGAACACTGCGATCGCCAAGAAAGGCAGCGAAGTGGAGAGCGTGAAAGAGTTTCTAGCCAAAGCCA AAGAAGACTTTCTGAGGAAATGGGAGAACCCTCCCCCGAGTAATGCTGGGCTTGAGGATTTTGAGAGGAA GAAAACCCTCGGGACGGGTTCCTTTGGAAGAGTCATGTTGGTGAAGCATAAAGCCACTGAGCAGTACTAC GCCATGAAGATCTTAGACAAGCAGAAGGTTGTTAAGCTGAAGCAAATAGAGCACACTCTGAATGAGAAGA GAATCCTGCAGGCCGTGGAGTTCCCGTTCCTTGTGCGGCTGGAGTACTCTTTTAAGGATAATTCTAATTT ATACATGGTTATGGAATACGTCCCTGGGGGAGAGATGTTCTCACATCTGAGAAGAATTGGAAGGTTCAGT GAGCCCCACGCCCGTTTCTATGCAGCCCAGATTGTGCTAACATTTGAGTACCTTCATTCCCTCGACCTCA TCTACAGAGATCTCAAGCCGGAAAACCTCTTAATTGACCACCAGGGTTACATCCAGGTCACAGATTTCGG GTTCGCCAAAAGAGTCAAGGGCAGGACATGGACATTGTGTGGCACCCCAGAGTACCTGGCCCCGGAGATC ATCCTCAGCAAGGGTTACAATAAGGCGGTGGACTGGTGGGCACTGGGCGTGCTGATCTATGAGATGGCTG CTGGCTACCCTCCATTCTTTGCTGACCAGCCAATTCAGATCTATGAGAAGATTGTCTCTGGAAAGGTCCG GTTCCCATCACACTTCAGCTCCGATCTCAAGGACCTTCTGCGGAACCTGCTGCAGGTGGATCTGACAAAG CGATTCGGGAACCTGAAGAACGGCGTGAGTGACATAAAGACCCACAAGTGGTTTGCCACAACTGACTGGA TTGCTATTTATCAGAGAAAGGTTGAGGCTCCATTCATACCAAAGTTCAGAGGCTCTGGCGATACCAGCAA CTTCGATGACTATGAAGAAGAAGAAATCCGTGTGTCTATAACAGAAAAATGTGGAAAGGAATTTTGTGAA TTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG205329 representing NM_011100
Red=Cloning site Green=Tags(s) MGNTAIAKKGSEVESVKEFLAKAKEDFLRKWENPPPSNAGLEDFERKKTLGTGSFGRVMLVKHKATEQYY AMKILDKQKVVKLKQIEHTLNEKRILQAVEFPFLVRLEYSFKDNSNLYMVMEYVPGGEMFSHLRRIGRFS EPHARFYAAQIVLTFEYLHSLDLIYRDLKPENLLIDHQGYIQVTDFGFAKRVKGRTWTLCGTPEYLAPEI ILSKGYNKAVDWWALGVLIYEMAAGYPPFFADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTK RFGNLKNGVSDIKTHKWFATTDWIAIYQRKVEAPFIPKFRGSGDTSNFDDYEEEEIRVSITEKCGKEFCE F TRTRPLE - GFP Tag - V |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
AscI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011100 |
| ORF Size | 1053 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011100.4, NP_035230.1 |
| RefSeq Size | 4341 bp |
| RefSeq ORF | 1056 bp |
| Locus ID | 18749 |
| UniProt ID | P68181 |
| Gene Summary | Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux (PubMed:9368018). Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates GPKOW which regulates its ability to bind RNA (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC209176 | Prkacb (untagged) - Mouse protein kinase, cAMP dependent, catalytic, beta (Prkacb), transcript variant 1, (10ug) |
CNY 3,990.00 |
|
| MR205329 | Prkacb (Myc-DDK-tagged) - Mouse protein kinase, cAMP dependent, catalytic, beta (Prkacb), transcript variant 1 |
CNY 5,856.00 |
|
| MR205329L1 | Lenti ORF clone of Prkacb (Myc-DDK-tagged) - Mouse protein kinase, cAMP dependent, catalytic, beta (Prkacb), transcript variant 1 |
CNY 5,890.00 |
|
| MR205329L2 | Lenti ORF clone of Prkacb (mGFP-tagged) - Mouse protein kinase, cAMP dependent, catalytic, beta (Prkacb), transcript variant 1 |
CNY 8,256.00 |
|
| MR205329L3 | Lenti ORF clone of Prkacb (Myc-DDK-tagged) - Mouse protein kinase, cAMP dependent, catalytic, beta (Prkacb), transcript variant 1 |
CNY 5,890.00 |
|
| MR205329L4 | Lenti ORF clone of Prkacb (mGFP-tagged) - Mouse protein kinase, cAMP dependent, catalytic, beta (Prkacb), transcript variant 1 |
CNY 5,890.00 |
