Brca2 (NM_001081001) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Fancd1; RAB163
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229768 representing NM_001081001 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCGTTGAATACAAAAGGAGACCAACTTTTTGGGAAATTTTTAAGGCGAGATGCAGCACAGCAGATT TAGGACCGATAAGCCTCAATTGGTTTGAGGAGCTTTCCTCAGAAGCCCCCCCATACAATTCTGAACCTCC GGAGGAATCTGAGTATAAGCCCCACGGTTATGAACCACAGCTGTTTAAAACACCACAGAGGAATCCCCCC TACCATCAGTTTGCTTCAACTCCAATAATGTTCAAAGAACGAAGTCAAACTCTACCACTGGACCAGTCGC CTTTCAGAGAATTAGGGAAGGTTGTTGCAAGTAGTAAACATAAAACTCACAGCAAAAAGAAGACCAAAGT GGACCCTGTGGTAGATGTTGCTAGTCCGCCTCTGAAATCTTGTCTCAGTGAAAGCCCTCTTACTCTGCGG TGCACACAGGCAGTACTACAACGAGAAAAGCCAGTGGTATCTGGAAGTTTATTTTATACACCAAAACTCA AGGAGGGTCAGACACCAAAACCTATTTCTGAGAGTCTGGGAGTTGAAGTGGATCCTGATATGTCTTGGAC AAGCTCATTAGCTACACCACCAACCCTTAGTTCCACTGTGCTCATAGCCCGAGATGAAGAAGCACGCAGC TCTGTAACGCCTGCTGACTCTCCCGCTACTTTGAAAAGCTGTTTTTCTAACCACAATGAAAGTCCACAAA AAAATGATAGATCTGTTCCCTCAGTGATTGACAGTGAAAACAAAAACCAGCAAGAAGCTTTTAGTCAGGG TTTGGGGAAAATGTTAGGAGATTCATCTGGCAAAAGAAATAGCTTCAAAGACTGCCTTAGAAAGCCAATA CCAAATATTCTAGAAGATGGGGAGACAGCTGTTGATACTTCCGAAGAAGATAGTTTCTCATTATGTTTTC CTAAGCGTAGAACCAGAAATCTACAGAAAATGAGAATGGGCAAGACAAGGAAAAAAATTTTCAGTGAAAC AAGAACTGATGAATTAAGCGAAGAAGCTAGAAGACAAACTGATGATAAAAACTCATTTGTATTTGAAATG GAACTAAGAGAGAGTGATCCTTTAGATCCAGGTGTAACAAGCCAGAAGCCCTTTTACAGCCAGAATGAGG AAATCTGCAATGAAGCTGTACAGTGTTCAGATAGTAGATGGTCTCAGTCAAACCTTTCTGGTCTAAATGA AACCCAGACAGGAAAAATAACCTTACCTCATATTTCTTCTCATAGCCAAAATATTTCAGAAGACTTCATA GATATGAAGAAAGAAGGTACTGGCTCTATTACTTCAGAAAAATCTTTACCTCATATTTCTAGTCTTCCAG AACCGGAAAAGATGTTCAGTGAGGAGACTGTGGTAGATAAGGAACATGAAGGACAGCATTTTGAATCACT TGAAGACTCCATTGCAGGGAAGCAAATGGTGTCTAGAACAAGTCAAGCAGCCTGTCTTTCTCCGAGTATC AGGAAGTCTATATTCAAGATGAGAGAGCCACTTGACGAGACTTTGGGTACTGTTTTCTCAGATAGTATGA CCAACTCAACCTTTACAGAAGAACATGAAGCCTCTGCATGTGGATTGGGAATACTTACTGCATGCTCACA GAGAGAGGATTCTATATGTCCTAGTTCAGTTGACACTGGAAGCTGGCCAACCACTCTCACTGACACTTCT GCCACTGTGAAAAATGCAGGTTTGATATCCACTCTAAAAAATAAAAAAAGAAAGTTTATTTACTCTGTAA GTGATGATGCATCTCTTCAAGGAAAAAAACTACAGACACACAGACAGTTAGAGCTTACTAATCTTTCAGC CCAGCTTGAAGCAAGTGCTTTTGAAGTACCATTGACCTTTACAAATGTAAATTCAGGTATACCAGATTCT TCTGACAAAAAACGCTGTTTACCGAATGATCCTGAAGAGCCATCCTTGACCAACTCTTTTGGGACTGCTA CCAGTAAAGAAATTAGTTATATTCATGCATTGATATCTCAGGATCTTAATGACAAAGAAGCAATAGTCAT TGAAGAAAAACCACAGCCATATACAGCCCGAGAAGCTGACTTTCTGTTATGCTTGCCAGAAAGAACATGT GAAAATGACCAAAAAAGTCCAAAAGTTTCCAATGGAAAAGAAAAAGTCTTAGTTTCAGCATGTCTTCCTT CAGCAGTGCAACTCAGCAGCATTAGCTTTGAATCACAGGAAAACCCTCTTGGTGACCACAACGGAACAAG TACTCTTAAATTAACTCCCAGCTCAAAGCTACCTCTGTCAAAGGCAGACATGGTTTCTAGAGAGAAAATG TGTAAAATGCCAGAGAAACTGCAATGTGAGAGTTGTAAAGTTAATATTGAACTAAGTAAAAACATCTTGG AGGTAAATGAAATATGTATCTTAAGTGAAAATTCTAAAACTCCTGGGCTTCTGCCACCTGGTGAAAATAT AATAGAAGTGGCCTCTTCAATGAAGTCACAGTTCAATCAAAATGCAAAAATAGTCATACAGAAGGACCAA AAAGGCTCACCTTTTATTTCAGAAGTAGCAGTCAATATGAACTCTGAAGAACTTTTCCCAGACAGTGGGA ATAATTTTGCTTTTCAAGTAACTAATAAATGCAATAAGCCTGATTTAGGAAGTTCAGTGGAACTCCAGGA AGAAGACCTCAGCCACACACAAGGGCCTAGTCTCAAGAACTCTCCCATGGCAGTAGATGAAGATGTAGAT GATGCGCATGCAGCCCAAGTGTTGATTACAAAGGACTCAGATTCATTAGCTGTGGTCCATGATTATACAG AGAAGAGCAGAAATAATATAGAGCAGCACCAGAAAGGAACCGAAGACAAAGATTTCAAGTCAAATTCCTC CTTGAATATGAAATCAGATGGGAACAGTGATTGTTCAGACAAATGGTCAGAGTTCTTGGATCCAGTCTTG AACCATAATTTTGGAGGTAGCTTCAGAACAGCTTCCAATAAAGAAATAAAACTTTCAGAGCATAATGTCA AGAAAAGTAAAATGTTCTTCAAAGATATTGAAGAACAGTATCCTACTAGGTTAGCTTGTATTGACATTGT TAATACCCTTCCATTAGCAAACCAGAAGAAACTAAGTGAACCTCATATATTTGATTTGAAATCAGTTACT ACTGTATCTACACAGTCTCACAATCAATCATCTGTTTCTCATGAAGATACTGACACAGCACCTCAGATGT TATCTTCAAAGCAAGATTTTCATTCAAATAATTTAACGACCAGCCAAAAAGCAGAAATTACAGAACTGTC TACTATTTTGGAAGAATCAGGAAGTCAGTTTGAATTCACACAGTTCAGAAAGCCAAGCCACATAGCACAG AATACATCTGAAGTGCCTGGAAACCAGATGGTTGTTTTAAGTACCGCTTCTAAGGAGTGGAAAGATACTG ATCTTCACCTCCCAGTGGATCCCTCTGTAGGTCAGACAGATCACAGCAAGCAATTTGAAGGTTCGGCTGG AGTTAAACAAAGCTTTCCTCACCTGTTAGAAGACACTTGTAACAAAAATACATCTTGTTTTTTACCAAAT ATAAATGAAATGGAGTTTGGAGGATTTTGTTCTGCTCTTGGCACAAAACTTAGTGTGTCTAATGAGGCTC TGAGAAAAGCTATGAAACTGTTCAGTGACATTGAAAATAGTGAGGAGCCTTCTGCAAAAGTAGGACCAAG AGGATTCTCTTCAAGTGCACACCATGATTCTGTTGCTTCAGTGTTTAAGATAAAGAAACAAAACACTGAA AAAAGTTTTGATGAAAAATCTAGTAAGTGCCAAGTAACATTACAAAATAATATTGAAATGACTACCTGTA TTTTTGTTGGCAGAAATCCTGAAAAATACATAAAGAATACAAAACATGAAGATAGCTATACTAGCTCTCA AAGAAATAATTTAGAAAACTCTGATGGTAGTATGTCAAGTACAAGTGGCCCAGTTTATATTCATAAAGGT GACAGTGATTTACCTGCCGATCAAGGCAGTAAGTGTCCTGAGTCATGTACCCAATATGCGAGAGAGGAAA ACACACAAATTAAGGAAAATATATCAGATTTAACATGTTTGGAAATTATGAAAGCTGAGGAAACATGTAT GAAATCTTCAGATAAAAAACAATTACCTTCAGATAAGATGGAACAAAATATAAAAGAGTTTAATATATCC TTTCAGACTGCAAGTGGGAAAAATACCAGAGTCTCCAAAGAGTCATTAAATAAAAGTGTGAATATTTTTA ATCGGGAAACAGATGAATTGACTGTCATTTCAGATTCTTTGAATTCTAAAATTCTCCATGGCATAAATAA GGACAAAATGCATACTTCATGTCACAAGAAAGCAATCAGTATTAAAAAGGTATTTGAAGACCATTTCCCA ATTGTAACTGTCAGTCAATTACCAGCTCAGCAGCATCCTGAATATGAAATAGAAAGTACCAAAGAACCTA CTCTGTTGAGTTTTCATACAGCTAGTGGGAAAAAAGTCAAAATTATGCAGGAATCTTTGGACAAAGTGAA AAACCTTTTTGATGAGACACAATATGTTAGGAAAACTGCCAGTTTTAGTCAAGGATCAAAACCCCTGAAG GACAGTAAAAAAGAACTTACATTAGCATATGAGAAAATTGAAGTAACTGCCTCAAAATGTGAAGAAATGC AGAACTTTGTCTCTAAGGAGACTGAAATGCTACCTCAGCAAAATTATCATATGTATAGGCAAACTGAAAA TCTCAAAACATCAAATGGTACCTCTTCCAAAGTACAAGAAAACATAGAAAATAATGTAGAAAAGAATCCT AGAATTTGCTGTATTTGTCAGTCCTCTTACCCAGTCACTGAAGATTCTGCTTTGGCATATTATACGGAGG ACAGTAGGAAAACTTGTGTCAGAGAGTCTTCTCTATCCAAAGGCAGAAAATGGCTTAGAGAACAGGGTGA TAAGCTTGGAACAAGAAATACTATCAAAATTGAGTGTGTAAAGGAACACACAGAAGATTTTGCAGGAAAT GCCTCATATGAACATAGTTTGGTCATTATCAGAACTGAAATTGATACAAATCATGTCTCTGAAAACCAAG TGTCAACCCTCCTTAGTGACCCTAATGTGTGTCATAGCTATCTATCCCAGTCTAGTTTTTGTCATTGTGA TGACATGCATAATGATTCAGGATATTTCTTAAAAAATAAAATTGATTCTGACGTTCCGCCAGACATGAAG AATGCTGAAGGCAATACCATTTCCCCCAGAGTATCTGCTACAAAAGAAAGAAATCTACACCCACAAACTA TAAATGAATATTGTGTTCAGAAACTGGAGACTAATACTTCACCACATGCAAATAAAGATGTAGCCATTGA CCCATCTCTGCTGGATTCAAGGAATTGTAAGGTAGGCTCACTCGTGTTTATTACAGCTCATTCACAAGAA ACTGAAAGAACAAAAGAGATAGTCACAGATAACTGTTATAAAATAGTTGAGCAAAACAGACAGAGTAAAC CAGACACTTGCCAGACAAGCTGTCATAAAGTATTGGATGATTCAAAGGATTTTATATGTCCTAGCTCTTC AGGTGATGTCTGCATAAACTCACGTAAGGATAGTTTTTGTCCTCATAATGAACAAATTTTACAACATAAC CAAAGTATGTCTGGACTGAAGAAAGCTGCAACACCACCTGTTGGTTTGGAAACTTGGGATACAAGTAAAT CTATAAGAGAACCTCCCCAGGCAGCCCATCCTTCACGCACTTATGGGATTTTTAGCACAGCAAGTGGAAA AGCTATACAAGTATCAGATGCTTCATTAGAAAAGGCAAGGCAAGTGTTTTCTGAGATGGATGGTGATGCT AAACAGTTATCTTCCATGGTGTCACTGGAAGGTAATGAAAAACCACATCACTCTGTGAAAAGAGAAAACT CTGTGGTGCATAGCACCCAGGGTGTATTGTCACTCCCAAAACCCCTCCCAGGCAATGTCAATTCATCTGT ATTCTCTGGATTTAGCACTGCAGGTGGAAAACTGGTCACAGTTTCAGAAAGTGCCTTACACAAAGTTAAA GGAATGTTAGAGGAGTTTGATTTGATCAGAACTGAACATACTCTCCAGCATTCACCTATACCTGAAGACG TATCAAAAATACTTCCTCAACCTTGTGCTGAAATCAGAACCCCAGAATACCCTGTAAACTCAAAATTGCA GAAAACCTACAATGATAAATCCAGCTTACCAAGTAATTATAAAGAAAGTGGTTCTTCGGGCAATACTCAA TCTATTGAAGTTTCTCTCCAACTCTCTCAGATGGAGAGAAACCAAGACACACAGTTGGTATTAGGAACAA AAGTATCCCATAGTAAGGCTAATCTTTTGGGAAAAGAACAAACTTTACCCCAAAACATAAAAGTAAAAAC TGATGAAATGAAAACATTTTCTGATGTTCCTGTGAAAACAAATGTAGGAGAGTATTACTCCAAAGAGTCA GAGAACTATTTTGAAACAGAAGCAGTGGAGAGTGCCAAAGCTTTTATGGAAGATGATGAGCTGACAGATT CTGAACAGACTCATGCCAAATGCTCACTGTTTACATGCCCCCAAAATGAGACTTTGTTTAATTCGAGAAC TAGGAAAAGAGGAGGAGTGACTGTTGACGCAGTTGGACAACCCCCAATCAAAAGAAGCTTATTAAATGAA TTTGACAGGATAATAGAAAGTAAAGGAAAATCCTTAACACCTTCAAAAAGCACTCCAGATGGCACAGTAA AAGACAGAAGCTTGTTCACACACCACATGTCTTTAGAGCCGGTTACCTGTGGACCCTTCTGCTCAAGTAA AGAAAGGCAAGGAGCCCAGCGCCCACATCTTACCTCACCTGCTCAAGAGCTTCTGTCTAAAGGGCATCCC TGGAGACACTCAGCTTTGGAAAAGTCTCCAAGCAGTCCTATAGTTTCAATCTTACCGGCTCACGACGTTT CTGCCACAAGAACCGAAAGGACCAGACATTCAGGCAAATCCACCAAAGTCTTCGTCCCGCCTTTCAAGAT GAAATCACAGTTTCACGGAGATGAACATTTCAATAGCAAGAACGTTAATTTGGAGGGAAAGAACCAAAAG AGCACAGATGGAGACAGGGAAGACGGGAACGACAGCCATGTTCGTCAGTTTAACAAAGATTTAATGTCAA GCCTTCAGAGTGCCAGAGACCTACAGGATATGCGAATTAAAAACAAAGAAAGGCGACATCTCCGTCTGCA GCCAGGCAGTCTGTATCTTACAAAGTCATCCACCCTGCCCCGGATCTCCCTGCAGGCTGCAGTAGGAGAC AGGGCTCCTTCTGCATGTTCTCCTAAGCAGCTCTATATATACGGCGTTTCTAAAGAGTGCATAAACGTTA ATAGCAAAAATGCTGAGTATTTTCAGTTTGATATTCAGGATCATTTTGGTAAAGAAGATCTGTGTGCTGG CAAAGGCTTTCAGTTGGCAGATGGTGGATGGCTGATCCCCTCCAATGATGGCAAGGCTGGAAAAGAAGAA TTTTATAGGGCTCTGTGTGACACTCCAGGTGTGGATCCAAAGCTTATTTCTAGCATCTGGGTTGCTAACC ATTACAGGTGGATTGTATGGAAACTGGCAGCTATGGAATTTGCTTTTCCTAAGGAATTTGCTAATAGATG CCTAAACCCAGAAAGAGTGCTGCTTCAACTGAAATACAGATACGATGTGGAAATTGACAACAGCAGGAGA TCGGCTCTAAAGAAGATACTGGAGAGGGATGACACAGCTGCAAAAACCCTTGTTCTCTGTATTTCTGACA TCATTTCACCAAGCACAAAGGTGTCTGAAACTTCAGGCGGTAAAACTAGTGGTGAGGATGCCAACAAAGT GGACACGATCGAACTCACAGATGGGTGGTACGCTGTCAGGGCCCAGCTAGACCCTCCACTCATGGCTCTT GTAAAGAGTGGCAAACTGACTGTGGGTCAGAAGATCATTACTCAGGGAGCAGAGCTGGTGGGCTCTCCTG ATGCCTGTGCACCTCTGGAAGCCCCAGACTCCCTAAGGCTAAAGATTTCTGCAAACAGCACAAGGCCTGC TCGCTGGCACAGCAGGCTGGGGTTCTTTCGTGACCCCAGGCCCTTCCCTCTGCCCTTGTCCTCACTGTTC AGTGACGGAGGAAATGTTGGCTGTGTGGATATCATTGTTCAGAGAGTGTACCCTTTACAGTGGGTGGAGA AGACAGTGTCTGGATTGTACATATTTCGTAGTGAGAGAGAAGAGGAGAAGGAAGCACTGAGATTTGCAGA GGCCCAGCAGAAGAAACTAGAGGCCTTGTTCACCAAAGTCCACACAGAGTTTAAAGACCACGAAGAAGAC ACAACACAGCGGTGTGTGCTGTCCCGCACACTCACACGGCAGCAAGTCCATGCTCTGCAGGATGGAGCAG AGCTCTATGCAGCAGTGCAGTATGCATCAGACCCAGATCACCTTGAGGCTTGTTTCAGCGAAGAGCAGCT AAGAGCCTTGAACAACTACAGACAAATGCTGAATGATAAGAAACAAGCGCGGATCCAGTCAGAATTCCGG AAGGCCCTGGAATCCGCTGAGAAAGAGGAGGGTTTATCAAGGGATGTTACAACTGTGTGGAAGCTGCGGG TTACAAGCTACAAGAAAAAGGAAAAATCAGCTCTGTTGAGTATCTGGCGTCCATCATCAGACTTATCCTC CCTGTTAACAGAAGGAAAGAGATACAGAATCTATCATCTTGCAGTGTCAAAATCGAAGAGTAAATTTGAA CGGCCCAGCATCCAGTTAACAGCCACAAAAAGAACTCAGTATCAACAGCTGCCGGTTTCCAGTGAGACTT TACTCCAGGTTTACCAGCCCAGGGAGTCCCTTCACTTCAGCAGACTGTCAGACCCAGCCTTTCAGCCTCC TTGTTCTGAAGTGGATGTCGTAGGAGTTGTAGTTTCTGTTGTAAAACCGATAGGTCTTGCTCCTTTGGTC TACCTGTCAGACGAGTGCCTTAATTTATTAGTGGTGAAATTCGGGATAGACCTTAATGAAGACATAAAGC CACGTGTGCTAATTGCCGCAAGCAACCTCCAGTGCCAGCCAGAATCCACATCAGGAGTGCCGACCTTATT TGCTGGCCATTTTTCCATATTTTCTGCCAGTCCAAAGGAGGCCTACTTTCAGGAGAAAGTCAACAACTTG AAGCATGCTATTGAGAATATCGATACATTTTACAAGGAGGCAGAAAAGAAGCTTATACACGTGCTTGAGG GAGACAGTCCCAAGTGGTCCACACCGAACAAAGACCCCACCCGAGAGCCCCATGCAGCCTCCACTTGCTG TGCTTCAGATCTCCTTGGTTCAGGAGGTCAGTTCCTGAGGATTTCACCTACTGGTCAGCAAAGTTATCAA AGTCCTTTATCACACTGCACACTGAAAGGGAAGTCTATGCCCCTGGCTCACTCAGCCCAGATGGCAGCAA AGTCTTGGAGTGGAGAGAACGAGATTGATGACCCAAAAACCTGTCGAAAAAGAAGAGCCTTGGATTTCCT AAGTCGGCTGCCCTTACCGTCCCCGGTCAGTCCCATCTGTACCTTTGTCTCTCCAGCTGCACAGAAGGCT TTTCAGCCGCCACGGAGCTGTGGCACGAAATACGCAACACCTATTAAAAAAGAACCCAGTTCCCCGCGGA GGAGAACGCCATTTCAGAAGACCAGTGGCGTTTCTCTGCCGGACTGTGATTCAGTAGCTGATGAAGAACT TGCCTTGCTCAGCACCCAGGCCCTTACGCCTGACTCCGTGGGAGGAAATGAACAAGCGTTTCCCGGTGAT TCCACAAGGAACCCCCAGCCAGCACAGAGGCCAGACCAGCAAGTAGGGCCCAGGTCCAGGAAGGAGTCTC TCAGGGACTGTAGAGGCGACAGCAGTGAGAAATTAGCTGTTGAGTCTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001081001
Insert Size	9990 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001081001.2, NP_001074470.1
RefSeq Size	11134 bp
RefSeq ORF	9990 bp
Locus ID	12190
UniProt ID	P97929
Gene Summary	Involved in double-strand break repair and/or homologous recombination. Binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA (ssDNA). Acts by targeting RAD51 to ssDNA over double-stranded DNA, enabling RAD51 to displace replication protein-A (RPA) from ssDNA and stabilizing RAD51-ssDNA filaments by blocking ATP hydrolysis. Part of a PALB2-scaffolded HR complex containing RAD51C and which is thought to play a role in DNA repair by HR. May participate in S phase checkpoint activation. Binds selectively to ssDNA, and to ssDNA in tailed duplexes and replication fork structures. May play a role in the extension step after strand invasion at replication-dependent DNA double-strand breaks; together with PALB2 is involved in both POLH localization at collapsed replication forks and DNA polymerization activity. In concert with NPM1, regulates centrosome duplication. Interacts with the TREX-2 complex (transcription and export complex 2) subunits PCID2 and SEM1, and is required to prevent R-loop-associated DNA damage and thus transcription-associated genomic instability, independently of its known role in homologous recombination (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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