Fap (BC019190) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	SIMP
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC019190 CCACGCGTCCGCACAGATGCGGTGACCCACGCTGTGCAGTGAGAATCAGCTAACTTGCAAAAACATCTGG AAAAATGAAGACATGGCTGAAAACTGTCTTTGGAGTTACCACCCTGGCTGCGCTTGCTTTAGTGGTGATA TGCATTGTCTTACGTCCCTCAAGAGTTTACAAACCTGAAGGAAACACAAAGAGAGCTCTTACCTTGAAGG ATATTTTAAATGGAACATTCTCATATAAAACATATTTTCCCAACTGGATTTCAGAACAAGAATATCTTCA TCAATCTGAGGATGATAACATAGTATTTTATAATATTGAAACAAGAGAATCATATATCATTTTGAGTAAT AGCACCATGAAAAGTGTGAATGCTACAGATTATGGTTTGTCACCTGATCGGCAATTTGTGTATCTAGAAA GTGATTATTCAAAGCTCTGGCGATATTCATACACAGCGACATACTACATCTACGACCTTCAGAATGGGGA ATTTGTAAGAGGATACGAGCTCCCTCGTCCAATTCAGTATCTATGCTGGTCGCCTGTTGGGAGTAAATTA GCATATGTATATCAAAACAATATTTATTTGAAACAAAGACCAGGAGATCCACCTTTTCAAATAACTTATA CTGGAAGAGAAAATAGAATATTTAATGGAATACCAGACTGGGTTTATGAAGAGGAAATGCTTGCCACAAA ATATGCTCTTTGGTGGTCTCCAGATGGAAAATTTTTGGCATATGTAGAATTTAATGATTCAGATATACCA ATTATTGCCTATTCTTATTATGGTGATGGACAGTATCCTAGAACTATAAATATTCCATATCCAAAGGCTG GGGCTAAGAATCCGGTTGTTCGTGTTTTTATTGTTGACACCACCTACCCTCACCACGTGGGCCCAATGGA AGTGCCAGTTCCAGAAATGATAGCCTCAAGTGACTATTATTTCAGCTGGCTCACATGGGTGTCCAGTGAA CGAGTATGCTTGCAGTGGCTAAAAAGAGTGCAGAATGTCTCAGTCCTGTCTATATGTGATTTCAGGGAAG ACTGGCATGCATGGGAATGTCCAAAGAACCAGGAGCATGTAGAAGAAAGCAGAACAGGATGGGCTGGTGG ATTCTTTGTTTCGACACCAGCTTTTAGCCAGGATGCCACTTCTTACTACAAAATATTTAGCGACAAGGAT GGTTACAAACATATTCACTACATCAAAGACACTGTGGAAAATGCTATTCAAATTACAAGTGGCAAGTGGG AGGCCATATATATATTCCGCGTAACACAGGATTCACTGTTTTATTCTAGCAATGAATTTGAAGGTTACCC TGGAAGAAGAAACATCTACAGAATTAGCATTGGAAACTCTCCTCCGAGCAAGAAGTGTGTTACTTGCCAT CTAAGGAAAGAAAGGTGCCAATATTACACAGCAAGTTTCAGCTACAAAGCCAAGTACTATGCACTCGTCT GCTATGGCCCTGGCCTCCCCATTTCCACCCTCCATGATGGCCGCACAGACCAAGAAATACAAGTATTAGA AGAAAACAAAGAACTGGAAAATTCTCTGAGAAATATCCAGCTGCCTAAAGTGGAGATTAAGAAGCTCAAA GACGGGGGACTGACTTTCTGGTACAAGATGATTCTGCCTCCTCAGTTTGACAGATCAAAGAAGTACCCTT TGCTAATTCAAGTGTATGGTGGTCCTTGTAGCCAGAGTGTTAAGTCTGTGTTTGCTGTTAATTGGATAAC TTATCTCGCAAGTAAGGAGGGGATAGTCATTGCCCTGGTAGATGGTCGGGGCACTGCTTTCCAAGGTGAC AAATTCCTGCATGCCGTGTATCGAAAACTGGGTGTATATGAAGTTGAGGACCAGCTCACAGCTGTCAGAA AATTCATAGAAATGGGTTTCATTGATGAAGAAAGAATAGCCATATGGGGCTGGTCCTACGGAGGTTATGT TTCATCCCTGGCCCTTGCATCTGGAACTGGTCTTTTCAAATGTGGCATAGCAGTGGCTCCAGTCTCCAGC TGGGAATATTACGCATCTATCTACTCAGAGAGATTCATGGGCCTCCCAACAAAGGACGACAATCTCGAAC ACTATAAAAATTCAACTGTGATGGCAAGAGCAGAATATTTCAGAAATGTAGACTATCTTCTCATCCACGG AACAGCAGATGATAATGTGCACTTTCAGAACTCAGCACAGATTGCTAAAGCTTTGGTTAATGCACAAGTG GATTTCCAGGCGATGTGGTACTCTGACCAGAACCATGGTATATTATCTGGGCGCTCCCAGAATCATTTAT ATACCCACATGACGCACTTCCTCAAGCAATGCTTTTCTTTATCAGACTGAACCAATGCAAGTACTAGCGT GTAGGACAGTCTGAAGGCTTCACATGGACGACTTGGACCATGAATATTGTAAAAGCCGCTAAAAATTTAG GAGGGCTCAGGAGGTTAGGCTCGGTATTGTTTACATTTCCCTATGCTCTGTGAAAGAAGAGAAAAGGGGA GCCACACATCTTGCTTTGGACACAATGTTTTATCACCTGTTCATTGCCGGGTAGCGGAAATAAAGTCAGA TGCTCAGGTTAAAAAAAAAAAAAAA
Restriction Sites	EcoRI-NotI
ACCN	BC019190
Insert Size	2286 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC019190, AAH19190
RefSeq Size	2615 bp
RefSeq ORF	2286 bp
Locus ID	14089
Gene Summary	This gene belongs to the serine protease family. The encoded protein is an inducible cell-surface bound glycoprotein specifically expressed in tumor-associated fibroblasts and pericytes of epithelial tumors and has protease and gelatinase activity. The protein plays a role in remodeling of the extracellular matrix (ECM) and may affect tumorigenesis and tissue repair. Alternately spliced transcript variants of this gene are described in the literature (PMID 9139873), but the full-length sequence of these variants is not available. [provided by RefSeq, Apr 2013]

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