Leprot (BC010289) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	MGC6694, LEPR, VPS55, OB-RGRP
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC010289 GCCCCAGCTCCAGAGACATGGCGGGCGTTAAAGCTCTCGTGGCGCTCTCCTTCAGTGGGGCAATTGGGCT GACTTTTCTTATGCTGGGATGTGCGTTGGAGGACTATGGGGTCACATATGACTCCGACGCGACCAGCAGT GCCTGTCGGGAACTGGCGTATTTCTTCACCACTGGGATTGTTGTTTCTGCCTTTGGACTTCCTGTTGTTC TTGCTCGTGTGGGTGTGATCAAGTGGGGAGCCTGTGGTCTTGTGCTGGCCGGCAACGCTGTCATTTTCCT CACAATTCAAGGCTTCTTCCTCGTCTTTGGAAGAGGAGATGATTTCAGTTGGGAACAGTGGTAGCCCCTC CTCCCCATGGAAAGCATTGAGTCTTAGAATTTGTACTGTACGTATATGTGTGCACATGTGTTTCAGATTT GAATATGCTGAGTTTTTTTATACCTTTATATCATGATCATCTTAGGGAAGATGTTGTAAACAAAAGATGA GTTTTACTCCTGGCACACAGGTCACTCCATTGACTCAAAGTGAATTATTTTTATCTTCTAGCTTCCAGAA AGCCTGTTACTATGGCCTTGTAGACAGCTGCTCACTTATCCAGGGGGTCTAAACCTTTTGCCTTGGGTTA GGCTTGGGAGACACTGGGGCAGATCTCCTACAGCCCAAACATAGGTGGCGCTCTCCCTGTACACTTAGAC TCATCTCATCCACACAGAGCATCAGCATCTGCTAGGAGCCCATTGGGGATGCAGGCTCAGACTGGCTAAC TTTGATGCGATTGCACACTTGGCACATGCACATTGACGGGTGAGGAGGTTTGTTGTAGAGGAATAAGCAT CATAGCTCCCCTGCCCTCTCTGGGTGTTGCATGCTCATTTGCAGGAAAAAAAAAAAATCACCCGCCCCCT GCTGTGAATGAGTTCAGACTTCAGGTGTTTCTAACATGTTCAAGTCTATAATACTTGTGTTGTTCCTCAA ATTTGGGGGACCTGTTCTTAGGCAGGAACAGATAAGTCATCCAAAAGGCAGTGGCTGATTGCTCAGAATA AGATACAAAATAACCAAAAAACCCCTCTGGGGTGCAAGGAGGGAAACTTGGCTGGAATGGTCCCTACTTT AAGTGGAGGGCAGGATAGACAATTCTTGGAAATGTCCCTCTACGGTGCCTCCCATCCCCCATGCAGCATT CCTGTTTGCTCTTGGTGGTGGGTCTCCTGAAGGGTATGAGGCAAGAGTGAGCAGAGAGGAGGGAGTCACT GAAGTTACACGCTGGTGCACTTTTCATCCTTGATATTGAACTTGATGTCACCTCCACAGGACACACAGGG ATCCACATCATTTTGGTTCTTATAAACAGTTTTTCTAGCTCATCTTTTTTATCTGGAGCCTACTGGGAGA AGGAAGTGAGAACTACAAGCACCCAGTGAGGATGCCATCAGCGGTGTTTAAGCATTGATGCTGCTCGCTA ATGAGTGTCCAGAGCACTCTCCCTTGAGGCTTATGGTCCAAGGAGTCAGCATCCGGGCCAGGGCTGGTGT GACGCTGGGACCAGCAGGTGAATGGACAGAGGACAGTGTGCAATGTTATATTTTGGCCTTGTGCATGACA TTATGTTTTGAATATTTTGTGTACAAATAGAATTGTTAGCGTTGTCATTTCTAGTATTTACATTAGAAAA ATTATTTAGATACTTGATCATTTAACTGGCATTTTTAATCATGACACTTGCTATTGTAATAAATTTGACT TTTAACACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC010289
Insert Size	327 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC010289, AAH10289
RefSeq Size	1815 bp
RefSeq ORF	327 bp
Locus ID	230514
Gene Summary	Negatively regulates leptin receptor (LEPR) cell surface expression, and thus decreases response to leptin. Negatively regulates growth hormone (GH) receptor cell surface expression in liver. May play a role in liver resistance to GH during periods of reduced nutrient availability.[UniProtKB/Swiss-Prot Function]

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