Human MMP-9 ELISA Kit (48-well)

CAT#: EA100105

Human MMP-9 ELISA Kit (48-well)

Size: 1 x 48 wells 1 x 96 wells



CNY 2,630.00


货期*
3周

规格
    • 1 x 48 wells

Product images

Specifications

Product Data
Description Human MMP-9 ELISA Kit (48-well)
Size 1 x 48 wells
Format 48-well strip plate
Assay Type Solid Phase Sandwich ELISA
Assay Length 3 hours
Signal Colorimetric
Curve Range 62.5-4000pg/ml
Sample Type Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume 20 uL
Specificity Natural and recombinant Human MMP-9 Ligand
Sensitivity 31pg/mL
Reactivity Human
Interference No significant interference observed with available related molecules.
Components
  • Aluminium pouches with a Microwell Plate coated with antibody to human MMP-9 (8x6)
  • 1 vials human MMP-9 Standard lyophilized, 4000 pg/ml upon reconstitution
  • 1 vials concentrated Biotin-Conjugate anti-human MMP-9 antibody
  • 1 vials Streptavidin-HRP solution
  • 2 bottle Standard /sample Diluent
  • 1 bottle Biotin-Conjugate antibody Diluent
  • 1 bottle Streptavidin-HRP Diluent
  • 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  • 1 vial Substrate Solution
  • 1 vial Stop Solution
  • 2 pieces Adhesive Films
  • package insert
Background

Matrix metalloproteinases (MMPs) are a family of zinc-dependent endopeptidases that degrade extracellular matrix proteins . They are secreted as zymogens (Pro-MMPs) that are activated by a variety of proteinases or by reaction with organic mercurials. They are inhibited by specific tissue inhibitors of metalloproteinases (TIMPs) and by 2-macroglobulin . The regulation of MMP activity is important in tissue remodeling, inflammation, tumor growth and metastasis .

Human MMP-9 (also known as gelatinase B) is secreted as a 92 kDa zymogen . Cleavage of Pro-MMP-9 at or near residue 87 results in the active enzyme with a mass of approximately 82 kDa . MMP-9 has three fibronectin type II domains, a hemopexin-like domain and a proline-rich type V collagen-homologous domain . Pro-MMP-9 can be activated by MMP-3 or by certain bacterial proteinases . MMP-9 is inhibited by.2-macroglobulin or by TIMP-1 , which binds to Pro-MMP-9 as well as to active MMP-9 . In vitro treatment of Pro-MMP-9 with 4-aminophenylmercuric acid (APMA) produces not only the 82 kDa active enzyme but also a C-terminal truncated form of approximately 65 kDa with the activity comparable to that of the 82 kDa form .

Pro-MMP-9 is secreted by monocytes, macrophages, neutrophils, keratinocytes, fibroblasts, osteoclasts, chondrocytes, skeletal muscle satellite cells, endothelial cells, and various tumor cells(1, 7- 21). Pro-MMP-9 expression is upregulated by TGF-.1, IL-1., TGF-., PDGF-AB, TNF-., and IL-1. . Substrates for MMP-9 include denatured collagen type I (gelatin), native collagens type IV, V, VII, X and XI, fibrinogen, vitronectin, IL-1., and entactin, a molecule that bridges laminin and type IV collagen .

Gene Symbol MMP9
Standard Curve
Representative standard curve for MMP-9 ELISA. MMP-9 was diluted in serial two-fold steps in Sample Diluent.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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