Bovine IgM (Fc specific) Rabbit Polyclonal Antibody

Specifications

Product Data
Product Name	Bovine IgM (Fc specific) Rabbit Polyclonal Antibody
Applications	ELISA, ID, IF, IHC, IP
Recommend Dilution	Immunocytochemical and Immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. To demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases. To identify a specific antigen using an reference antibody of bovine origin known to be of the IgM isotype in the middle layer of the indirect test procedure. In non-isotopic assay methodology (e.g. ELISA) to measure IgM in Bovine serum or other body fluids. Recommneded Dilutions: 1/20-1/80.
Reactivity	Bovine
Host	Rabbit
Immunogen	Purified normal IgM isolated from pooled Bovine serum. Feund’s complete adjuvant is used in the first step of the immunization procedure.
Formulation	PBS, pH 7.2 without preservatives and foreign proteins Label: FITC State: Lyophilized hyperimmune IgG fraction Label: Fluorescein Isothiocyanate isomer 1 Absorption emission: 492 nm / 515 nm Molar radio: Fluorescein/IgG ~ 1.4
Concentration	10.0 mg/ml
Purification	Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Conjugation	FITC
Storage Condition	Store lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer. Avoid Repeated thawing and freezing.
Note	Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. Special attention is given to the removal of antibodies to common Ig/Fab. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

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