GRF2 (RAPGEF1) Mouse Monoclonal Antibody [Clone ID: UMAB140]

Specifications

Product Data
Clone Name	UMAB140
Applications	10k-ChIP, IF, IHC, WB
Recommend Dilution	IHC 1:100~200
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human RAPGEF1(NP_941372) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	Rap guanine nucleotide exchange factor 1
Database Link	NP_941372 Entrez Gene 107746 MouseEntrez Gene 63881 RatEntrez Gene 2889 Human UniProt ID Q13905
Background	This gene encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]
Synonyms	C3G; GRF2
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Focal adhesion, Insulin signaling pathway, Neurotrophin signaling pathway, Renal cell carcinoma

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