Vimentin (VIM) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI5D7]

Specifications

Product Data
Clone Name	OTI5D7
Applications	IF, IHC, WB
Recommend Dilution	WB 1:200 - 1:1000, IHC 1:150
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 210-466 of human VIM (NP_003371) produced in E.coli.
Formulation	PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration	0.5 mg/ml
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Biotin
Storage Condition	Store at -20°C as received.
Predicted Protein Size	53.5 kDa
Gene Name	vimentin
Database Link	NP_003371 Entrez Gene 22352 MouseEntrez Gene 81818 RatEntrez Gene 7431 Human UniProt ID P08670
Background	This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract. [provided by RefSeq, Jun 2009]
Synonyms	CTRCT30; HEL113
Reference Data
Protein Families	ES Cell Differentiation/IPS

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