KRT14 Rabbit Monoclonal Antibody [Clone ID: 2G4]

Specifications

Product Data
Clone Name	2G4
Applications	ELISA, IHC
Reactivity	Human
Modifications	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Host	Rabbit
Clonality	Monoclonal
Immunogen	The original antibody was generated by immunizing 4-6 weeks old female ICR mice with recombinant protein comprising amino acids 458-472 of cytokeratin 7 in equal volume of Freund's incomplete adjuvant.
Specificity	This antibody is capable of binding human cytokeratin 14. Cytokeratins are intermediate filaments found in the intracytoplasmic cytoskeleton of epithelial tissue. The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro. The binding characterization of this antibody was done using ELISA. The original IgG1 antibody binds cytokeratin 5/6 with a high affinity constant of 4.8× 10^(8) M^(-1). This antibody was also used in the immunohistochemical analysis of frozen paraffin sections of tonsil tissues and esophageal squamous cell carcinoma. The immunohistochemical staining pattern of this antibody is similar to commercially available antibody L1002 (CN113087793).
Formulation	PBS with 0.02% Proclin 300.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Database Link	UniProt ID P02533
Synonyms	CGMCC NO 20761; CK-14; CK 14; CK14; cytokeratin-14; K14; keratin-14; keratin type I cytoskeletal 14; KRT14; L1002
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.