H3C14 Rabbit Polyclonal Antibody

CAT#: TA397470

Histone H3 K4me2 Antibody



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CNY 8,363.00


货期*
4周

规格
    • 50 ug

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Specifications

Product Data
Applications ChIP, IF, IHC, WB
Recommend Dilution WB: 1:500
IHC: 1:50
IF: 1:50
CHiP: 2-5µg/million cells
Reactivity C. elegans, Human
Host Rabbit
Clonality Polyclonal
Immunogen Histone H3 [Dimethyl Lys4] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic dimethylated peptide surrounding Lysine 4 of human Histone H3.2.
Specificity Anti-Histone H3 [Dimethyl Lys4] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H3.2. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.
Formulation 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration 1.1 mg/ml - lot specific
Conjugation Unconjugated
Storage Condition Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Gene Name histone cluster 2, H3c
Background H3K4me2 is a modification thought to have a role in transcriptional memory. In CD4+ T lymphocytes, H3K4Me2 is present within gene bodies regulating cellular function, but not in those of housekeeping genes, which indicates that the modification has a role in refining the tissue-specificity of expressed genes. This type of cellular identity targeting is also noted in work with human and mouse spermatozoa; the H3K4Me2 modification marks genes that are relevant in spermatogenesis. Most effects of H3K4Me2 seem to be attributed to its transcriptional activation; however, recent work also indicates that it may also play an RNA-dependent regressive role, related to the GAL-1 promoter. Anti-Histone H3 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics Research.
Synonyms rabbit anti-Histone H3 dimethyl Lys4 antibody, H3.3B, H3.3AH3F3H3F3B, H3 histone, family 3A, histone H3.3, MGC87783, MGC87782, H3K4me2
Note Anti-Histone H3 [Dimethyl Lys4] antibody is tested in Western Blot, Immunofluorescence, Chromatin Immunoprecipitation, and Dot Blot. This antibody is useful in Immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.
Reference Data
Protein Pathways Systemic lupus erythematosus
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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