gox Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	WB: 1:3500 - 1:16,000 IHC: 1:2,000 - 1:8,000 ELISA: 1:35,000 - 1:160,000
Reactivity	Aspergillus niger
Host	Rabbit
Clonality	Polyclonal
Immunogen	Anti-Glucose Oxidase Antibody was produced by repeated immunizations with Aspergillus niger Glucose Oxidase protein.
Specificity	Anti-Glucose Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Glucose Oxidase [Aspergillus niger].  Cross reactivity against Glucose Oxidase from other tissues and species may occur but have not been specifically determined.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Method	Restore with deionized water (or equivalent) - Reconstitution Volume: 100 µL
Concentration	1.0 mg/ml - lot specific
Conjugation	Unconjugated
Storage Condition	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Database Link	UniProt ID P13006
Background	Anti-Glucose Oxidase detects glucose oxidase. Glucose oxidase enzyme (GOx) is an oxido-reductase that catalyzes the oxidation of glucose to hydrogen peroxide and D-glucono-δ-lactone. In cells, it aids in breaking the sugar down into its metabolites. Glucose oxidase is widely used for the determination of free glucose in body fluids, in vegetal raw material, and in the food industry. It also has many applications in biotechnologies, typically enzyme assays for biochemistry including biosensors in nanotechnologies. Anti-Glucose Oxidase Antibody is ideal for investigators involved in Cell Signaling and Signal Transduction research.
Synonyms	rabbit anti-Glucose Oxidase Antibody, Beta D Glucose Oxygen 1 Oxido Reductase antibody, Glucose oxidase (Precursor) antibody, Glucose Oxyhydrase antibody, GOD antibody
Note	Anti-Glucose Oxidase Antibody has been tested by ELISA and western blot and is suitable for IHC. Researchers should determine optimal titers for applications that are not stated below.
Reference Data

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