CTNNB1 Mouse Antibody [Clone ID: M118]

Specifications

Product Data
Clone Name	M118
Applications	ICC, IHC, IP, WB
Recommend Dilution	WB: 1:1000 ICC: 1:250
Reactivity	Human, Rat, Mouse
Host	Mouse
Immunogen	Clone (M118) was generated from a recombinant protein corresponding to amino acid residues in the C-terminal region of human β-Catenin. This peptide sequence is highly conserved in rat and mouse β-Catenin.
Specificity	The antibody detects a 92 kDa* protein corresponding to the molecular mass of β-Catenin on SDS-PAGE immunoblots of A431 and Hct116 src transformed cells. In addition, this antibody recognizes only β-Catenin in immunoprecipitations using anti-β-Catenin versus γ-Catenin.
Formulation	PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Concentration	lot specific
Purification	Protein A Purified
Conjugation	Unconjugated
Storage Condition	Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Predicted Protein Size	92
Database Link	UniProt ID P35222
Background	β-Catenin is a 92 kDa protein that binds to the cytoplasmic tail of E-Cadherin. The cadherins, transmembrane adhesion molecules, are found with catenins at adherens junctions. Deletions in the cytoplasmic domain of E-Cadherin eliminate catenin binding and result in a loss of cell adhesion. Tyrosine phosphorylation of β-Catenin can regulate its interaction with critical components of adherens junctions. Both Fer and Fyn kinases phosphorylate tyrosine 142 in vitro. Overexpression of these kinases in epithelial cells disrupts interactions between α- and β-Catenins. The phosphorylation of tyrosine 142 may act as a switch from the transcriptional to the adhesive role of β-Catenin. Src family kinases can also phosphorylate tyrosine 86 and 654 in β-Catenin. The Tyr-654 phosphorylation regulates β-Catenin binding to E-cadherin. Thus, site-specific tyrosine phosphorylation of β-Catenin may regulate protein-protein interactions leading to changes in cell adhesion.
Note	Protein G purified tissue culture supernatant.
Reference Data

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