Phospho-Cx35 (pSer276) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	WB: 1:1000
Reactivity	Fish, Mouse, Rabbit
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic phospho-peptide corresponding to amino acid residues of perch connexin 35/36 surrounding Ser276, conjugated to keyhole limpet hemocyanin (KLH).
Specificity	Specific for endogenous levels of the ~35 kDa connexin-35/36 protein phosphorylated at Ser276.
Formulation	10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Concentration	lot specific
Purification	Antigen Affinity Purified from Pooled Serum
Conjugation	Unconjugated
Storage Condition	Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Predicted Protein Size	35
Background	Connexin 35/36 is the most abundant gap junction protein in the central nervous system. Connexin gap junction coupling in the retina has been shown to be regulated by light exposure and neurotransmitter signaling, but more recently has been observed to also depend on direct phosphorylation of connexin (Ouyang et al., 2005, Patel et al., 2006). PKA phosphorylation of Ser110 in the intracellular loop and Ser276 in the C-terminal domain can cause uncoupling of connexin 35 gap junctions (Ouyang et al., 2005). The largest uncoupling effect is seen when both of these sites are phosphorylated, with a much smaller degree of uncoupling observed when only one is phosphorylated (Kothmann et al., 2007). C-terminal domain phosphorylation of connexin 35 at Ser276 is observed in teleost, amphibian, and avian species, with connexin 36 phosphorylation at Ser293 being the mammalian equivalent.
Note	Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
Reference Data

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