CD4 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	FC, ICC/IF, IHC, Simple Western, WB
Recommend Dilution	Dual RNAscope ISH-IHC, Immunocytochemistry/ Immunofluorescence: 1:50-1:200, Western Blot: 1 ug/ml, Simple Western: 1:100, Flow Cytometry: 5 ug/ml, Immunohistochemistry-Frozen, Immunohistochemistry: 1:50-1:500, Immunohistochemistry-Paraffin: 1:50-1:500
Reactivity	Human, Mouse, Rat, Rabbit
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide made to an C-terminal region of the human CD4 protein (within residues 400-458). [Swiss-Prot P01730]
Formulation	PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	CD4 molecule
Database Link	NP_000607 Entrez Gene 12504 MouseEntrez Gene 24932 RatEntrez Gene 920 Human UniProt ID P01730
Background	T-cell surface glycoprotein CD4 (CD4) encodes a membrane glycoprotein found on T lymphocytes. CD4 interacts with major histocompatibility complex (MHC) class II anti-genes and is also a receptor for the human immunodeficiency virus (HIV). Once the CD4 protein binds to the cell membrane of HIV-1 gp120 and P4HB/PDI it becomes part of P4HB/PDI-CD4-CXCR4-gp120 complex. The CD4 protein interacts with HIV-1 envelope protein, Vpu, human herpes virus 7 capsid proteins, and PTK2. In immune mediated and infectious diseases of the central nervous system, CD4 functions as an indirect mediator of neuronal damage. CD4 will also initiate or augment early phase T-cell activation. The CD4 gene is expressed in T lymphocytes, B cells, macrophages, granulocytes, and specific regions of the brain. There are three identified splice variants that encode different isoforms for CD4; one with a predicted transmembrane (TM) region and two with predicted signal peptides.
Synonyms	CD4mut
Note	This CD4 antibody is useful for Immunocytochemistry/Immunofluorescence, Flow (see reviews), Western Blot, and Immunohistochemistry-paraffin embedded sections. In WB, a band is seen ~45 kDa. In ICC/IF, membrane staining was observed in human brain cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. In ICC/IF, membrane staining was observed in Jurkat cells.
Reference Data
Protein Families	Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transmembrane
Protein Pathways	Antigen processing and presentation, Cell adhesion molecules (CAMs), Hematopoietic cell lineage, Primary immunodeficiency, T cell receptor signaling pathway

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.