Alkaline Phosphatase (ALPP) Mouse Monoclonal Antibody [Clone ID: 8B6]

Specifications

Product Data
Clone Name	8B6
Applications	ELISA, FC, ICC/IF, IHC, WB
Recommend Dilution	Flow Cytometry, Immunocytochemistry/ Immunofluorescence: 1:10-1:500, ELISA: 1:100-1:2000, Immunohistochemistry: 1:10-1:500, Western Blot: 1:1000, Immunohistochemistry-Paraffin: 1:100-1:200, Immunohistochemistry-Frozen: 1:10-1:500, Radioimmunodiffusion
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Hep-2 cells with boosted surface expression of Alkaline Phosphatase, Placental. [UniProt# P05187].
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein A purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	70 kDa
Gene Name	alkaline phosphatase, placental
Database Link	NP_001623 Entrez Gene 250 Human UniProt ID P05187
Background	Alkaline Phosphatase, Placental, also known as PLAP and ALPP is an alkaline phosphatase located on the second chromosome. Alkaline phosphatases are phosphodiesterases. The placental-specific isozyme of Alkaline Phosphatase is found in trophoblast cells of normal human mature placenta, seminomas of testis and ovarian carcinomas. The detection of alkaline phosphatase in serum is a marker for ovarian and testicular cancer (PMID: 21809268).
Synonyms	ALP; PALP; PLAP; PLAP-1
Note	This Alkaline Phosphatase, Placental Antibody (8B6) is useful for Immunohistochemistry on paraffin-embedded sections and frozen sections, Immunocytochemistry/Immunofluorescence, ELISA, Western blot and Radioimmunodiffusion. In WB a band can be seen at ~70 kDa. For IHC, Proteolytic Induced Epitope Retrieval (PIER) is required. Use in Flow Cytometry reported in various pieces of scientific literature
Reference Data
Protein Pathways	Folate biosynthesis, Metabolic pathways

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