Caspase 3 (CASP3) Mouse Monoclonal Antibody [Clone ID: 31A893]

Specifications

Product Data
Clone Name	31A893
Applications	ICC/IF, IHC, WB
Recommend Dilution	Immunocytochemistry/ Immunofluorescence, Western Blot: 2 ug/ml, Immunohistochemistry: 5 ug/mL, Immunohistochemistry-Paraffin: 5 ug/ml
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	A recombinant full-length human Caspase-3 protein was used as the immunogen for this antibody.
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	caspase 3
Database Link	NP_116786 Entrez Gene 836 Human UniProt ID P42574
Background	This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family and is a downstream effector cysteine protease in the apoptotic pathway. Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Gene for Caspase-3 also known as Yama, CPP32, and apopain codes for a 32-kDa protein (2-4). Caspase-3 cleaves the death substrate poly(ADP-ribose) polymerase (PARP) to a specific 85 kDa form observed during apoptosis and is inhibitable by the CrmA protein. Other Caspase-3 substrates include DNA-PK, actin, GAS2, and procaspase-6, etc. Caspase-3 is activated by cleavage events at Asp-28/Ser-29 (between N-terminal pro-domain) and Asp-175/Ser-176 (between large and small subunits) to generate a large subunit of 17-kDa and a small subunit of 12-kDa. The protein is ubiquitously expressed in normal human tissues including the liver, spleen, heart, liver and kidney.
Synonyms	CPP32; CPP32B; SCA-1
Note	For IHC-P validation of this target, 1M EDTA pH 9.0 buffer was used (citrate buffer ph 6.0 did not work)
Reference Data
Protein Families	Druggable Genome, ES Cell Differentiation/IPS, Protease
Protein Pathways	Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Colorectal cancer, Epithelial cell signaling in Helicobacter pylori infection, Huntington's disease, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, p53 signaling pathway, Parkinson's disease, Pathways in cancer, Viral myocarditis

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