TRPA1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, IP, WB
Recommend Dilution	WB: 1:200-1:2000; IHC: 1:100-1:3000
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide (C)NSTGIINETSDHSE, corresponding to amino acid residues 747-760 of human TRPA1. 1st extracellular loop.
Formulation	Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Reconstitution Method	Add 50 ul double distilled water (DDW) to the lyophilized powder.
Purification	Affinity purified on immobilized antigen.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	transient receptor potential cation channel subfamily A member 1
Database Link	NP_015628 Entrez Gene 277328 MouseEntrez Gene 312896 RatEntrez Gene 8989 Human UniProt ID O75762
Background	The TRPA family is comprised of only one mammalian member, the TRPA1 (formerly named ANKTM1). TRPA1 is expressed in peripheral sensory neurons, where it is suggested to contribute to the detection of painful stimuli. Originally it was thought that TRPA channels sensed painfully cold temperatures, but a more conservative description is that TRPA1 is sensitive to membrane/cytoskeletal perturbations by cold and perhaps stretch. In addition, it is sensitive to pungent natural compounds present in cinnamon oil, mustard oil, and wintergreen oil. TRPA1 is also expressed in hair cells, where its role in sensing mechanical forces is still unclear and controversial. TRPA1 has similar structure as all TRP ion channel members, six transmembrane domains, intracellular N-and C-terminus. However, its N-terminus domain possesses 17 ankyrin repeats that might indicate its potential role as a mechanosensor. TRPA1 is expressed in nociceptive neurons expressing TRPV1 as well and might serve as a marker for poly-modal nociceptors.
Synonyms	ANKTM1; FEPS
Note	This antibody was tested in live cell imaging. Please see IF/ICC data for detail.
Reference Data
Protein Families	Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane

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