SQSTM1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, IHC, WB
Recommend Dilution	WB: 0.5-2 µg/mL; IF: 20 µg/mL; IHC: 2-5 µg/mL;. Antibody validated: Western Blot in human and mouse samples; Immunofluorescence and Immunohistochemistry in human, mouse and rat samples. All other applications and species not yet tested.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	SQSTM1 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human SQSTM1. The immunogen is located within the last 50 amino acids of SQSTM1.
Formulation	PBS containing 0.02% sodium azide.
Purification	SQSTM1 Antibody is affinity chromatography purified via peptide column.
Conjugation	Unconjugated
Storage Condition	Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Gene Name	sequestosome 1
Database Link	NP_001135770 Entrez Gene 18412 MouseEntrez Gene 113894 RatEntrez Gene 8878 Human UniProt ID Q13501
Background	SQSTM1/p62 is an adapter protein which binds ubiquitin and regulates signaling cascades through ubiquitination. It may regulate the activation of NF-kappaB by TNF-alpha, nerve growth factor (NGF) and interleukin-1. SQSTM1/p62, a co-interacting protein of the atypical PKC isoforms, has a UBA domain at its C-terminal end, which binds non-covalently to polyubiquitin chain. SQSTM1’s UBA domain is necessary for recruitment of polyubiquitin and aggresome formation. SQSTM1 may play a role in titin/TTN downstream signaling in muscle cells and may be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels. Mutations in the ubiquitin-associated (UBA) domain of SQSTM1 commonly cause Paget’s disease of bone since the UBA is necessary for aggregate sequestration and cell survival.
Synonyms	A170; OSIL; p60; p62; p62B; PDB3; ZIP3
Reference Data
Protein Families	Druggable Genome, Transcription Factors

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