MHC Class II Rat Monoclonal Antibody [Clone ID: YKIX334.2]

Specifications

Product Data
Clone Name	YKIX334.2
Applications	FC, IHC, IP
Recommend Dilution	Immunoprecipitation. Immunohistochemistry on Frozen Sections. Flow Cytometry: Use 10 µl 1/50-1/100 diluted antibody to label 106 cells in 100 µl.
Reactivity	Canine
Host	Rat
Clonality	Monoclonal
Immunogen	Con A activated canine peripheral blood cells. Spleen cells from immunised DA rats were fused with cells of the rat Y3/Ag1.2.3 myeloma cell line.
Specificity	This antibody recognises monomorphic Canine MHC Class II and was classified at the First Canine Leucocyte Antigen Workshop. The antibody immunoprecipitates an antigen of 32/34kD and blocks the proliferation of MHC Class II dependent responses in vitro. In Dogs, MHC Class II is expressed by all peripheral blood mononuclear cells.
Formulation	PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Synonyms	MHC Class 2, HLA Class II
Reference Data

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