PCNA Mouse Monoclonal Antibody [Clone ID: PC10]

Specifications

Product Data
Clone Name	PC10
Applications	FC, IHC, IP, WB
Recommend Dilution	Flow Cytometry: Use 10 µl of 1/100 diluted antibody to label 10e6 cells in 100 µl. Cell permeabilisation required). Immunohistochemistry on Frozen Sections and Paraffin embedded sections: 1/50-1/100. It does not require antigen unmasking. Recommended Positive Control Tissue: Tonsil. Immunoprecipitation. Western blot (1/1000).
Reactivity	Amphibian, Drosophila, Fish, Mammalian, Yeast
Host	Mouse
Clonality	Monoclonal
Immunogen	Rat PCNA made in the protein A expression vector pR12T2.
Specificity	This antibody recognises PCNA, a nuclear protein vital for cellular DNA synthesis.
Formulation	PBS, pH 7.4, containing 0.09% Sodium Azide as preservative. State: Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	proliferating cell nuclear antigen
Database Link	UniProt ID P12004
Synonyms	Cyclin
Reference Data

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