CD55 / DAF Mouse Monoclonal Antibody [Clone ID: 67]

Specifications

Product Data
Clone Name	67
Applications	FC, IHC
Recommend Dilution	Flow Cytometry: Use 10 µl of 1/10-1/25 diluted antibody to label 10e6 cells in 100 µl. Immunohistochemistry on Frozen Sections: 1/100-1/1000. Recommended Positive Control Tissue: Human Tonsil. (Successful use in immunohistochemistry on paraffin embedded sections (1/50-1/100 using heat treatment for antigen retrieval) was reported by some researchers. As this detection method has not been verified by Acris Antibodies, the application was deleted from the database. This does not necessarily exclude the use in such procedure).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	K562 cells
Specificity	This antibody recognises the CD55 cell surface antigen. Erythrocyte expression is very weak. Please Note: We do not recommend the use of this reagent to stain Erythrocytes.
Formulation	PBS,pH 7.4, with 0.09% Sodium Azide as preservative. State: Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Affinity Chromatography on Protein G.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	CD55 molecule (Cromer blood group)
Database Link	Entrez Gene 1604 Human UniProt ID P08174
Background	CD55 is the complement regulatory protein, decay accelerating factor (DAF). It is a 70kD glycoprotein (in erythrocytes) anchored in the membrane by glycosylphosphatidylinositol tail. In other cells the apparent molecular weight is somewhat larger. It has a substantial content of O-glycans, and also on N-glycan. DAF binds to activated C4b or C3b complement fragments on the cell surface, preventing the assembly and accelerating the decay of both classical and alternative pathways. DAF carries the Cromer related blood group antigens. DAF has a wide distribution on cells in non-haemopoietic tissues, particularly epithelium and is specifically found at the foetal-maternal interface in placenta. Soluble forms of DAF are found, for example, in plasma, saliva and urine. The antigen on erythrocytes is pronase and chymotrypsin sensitive, but resistant to trypsin. CD55 is expressed by a wide range of cell types.
Synonyms	CR; CROM; DAF; TC
Reference Data

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