Carbonic Anhydrase II (CA2) Rabbit Polyclonal Antibody

CAT#: R1068HRPS

Carbonic Anhydrase II (CA2) rabbit polyclonal antibody, HRP, Purified



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CNY 7,688.00


货期*
4周

规格
    • 100 ug

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Specifications

Product Data
Applications ELISA, IHC, WB
Recommend Dilution Western blot: 1/250-1/1,000.
ELISA: 1/500-1/2,500.
This product has been assayed against 1.0 µg of Carbonic anhydrase 2 [human erythrocytes] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/1,000 to 1/6,000 of the reconstitution concentration is suggested.
Immunochemistry: Paraffin (FFPE) or Frozen sections; 1/100 suggested to begin.
Reactivity Human
Host Rabbit
Clonality Polyclonal
Immunogen Carbonic anhydrase 2 from human erythrocytes
Specificity This antibody detects human Carbonic anhydrase 2. Cross reactivity against Carbonic anhydrase 2 from other tissues and species may occur but have not been specifically determined.
Immunoelectrophoresis give a single precipitin arc against anti-peroxidase, anti-rabbit serum as well as  purified and partially purified Carbonic anhydrase 2 [human erythrocytes].
Formulation 0.02M Potassium phosphate, 0.15M Sodium chloride, pH 7.2
Label: HRP
State: Purified
State: Lyophilized Ig fraction
Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!)
Label: Horseradish peroxidase
Reconstitution Method Restore with 0.1 ml of deionized water (or equivalent).
Concentration lot specific
Purification Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation HRP
Storage Condition Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name carbonic anhydrase 2
Background Carbonic anhydrase II (CAII) is a single polypeptide chain of molecular weight 29kDa. It is present in the cytosol of most tissues, but highest concentrations are found, like Carbonic Anhydrase I, in erythrocytes. The concentration in erythrocytes is about 20µM. It catalyses the hydration of CO2 and the hydrolysis of esters. Zn2+ ion cofactor is required for enzyme activity. Certain point mutations occur without apparent clinical effect, however, complete absence leads to mild mental retardation and cerebral calcification, osteoporosis and renal tubular acidosis.
Synonyms Carbonic anhydrase II, Carbonate dehydratase II, Carbonic anhydrase C, CA2, CA-II, CAC
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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