EPCAM Mouse Monoclonal Antibody [Clone ID: Ber-EP4]

Specifications

Product Data
Clone Name	Ber-EP4
Applications	IHC
Recommend Dilution	Immunohistochemistry on Formalin-Fixed Paraffin Embedded Sections: Use at 1/50-1/150 dilution in an ABC method (30 minutes at RT). Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Recommended Positive Control: Kidney.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	BALB/C mice were immunized with human breast carcinoma cell line, MCF-7.
Specificity	Recognizes Human Epithelial Antigen. Other species not tested. Cellular Localization: Cell membrane.
Formulation	State: Supernatant State: Liquid Tissue Culture Supernatant Preservative: Less than 0.1% Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	epithelial cell adhesion molecule
Database Link	Entrez Gene 4072 Human UniProt ID P16422
Background	Epithelial antigen is cell surface glycoprotein and is broadly distributed in epithelial cells and displays a highly conserved expression in carcinomas. Epithelial antigen has been know to play an important role as tumor-cell marker in lymph nodes from patients with esophageal carcinoma. Epithelial antigen can be used to distinguish between basal cell and basosquamous carcinomas and squamous cell carcinoma of the skin.
Synonyms	Ep-CAM, Epithelial cell adhesion molecule, GA733-2, EGP314, KSA, TROP1, Trop-1, M1S2, M4S1, MIC18
Reference Data

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