Cytokeratin 10 (KRT10) Mouse Monoclonal Antibody [Clone ID: DE-K10]

Specifications

Product Data
Clone Name	DE-K10
Applications	IHC, WB
Recommend Dilution	Western Blot. Cell Suspensions. Immunohistochemistry on Frozen Sections: 1/5-1/10 (Fixation with acetone for 5 min. at -20°C is recommended). Immunohistochemistry on Paraffin Sections: 1/100 (Fixation with neutral formalin and a pretreatment with pronase or trypsin is also recommended).  Recommended Positive Control: Squamous cell carcinoma, epidermis. Incubation Time: 1 h at room temperature.
Reactivity	Canine, Feline, Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Cytoskeletal preparation from Human epidermis.
Specificity	This antibody specifically recognizes the 56.6 kDa Keratin K10 (formerly also designated Cytokeratin 10) polypeptide in squamous cells and carcinomas from e.g. epidermis, lung, bladder, cervix, oesophagus.
Formulation	0.15M PBS State: Supernatant State: Liquid Tissue Culture Supernatant Stabilizer: 1% BSA Preservative: 0.09% Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	keratin 10
Database Link	Entrez Gene 3858 Human UniProt ID P13645
Background	Cytokeratin 10 is a heterotetramer of two type I and two type II keratins. Cytokeratin 10 is generally associated with keratin 1. It is seen in all suprabasal cell layers including stratum corneum. A number of alleles are known that mainly differ in the Gly-rich region (positions 490-560). Defects in cytokeratin 10 are a cause of epidermolytic hyperkeratosis (EHK), also known as bullous congenital ichthyosiform erythroderma (BCIE) or bullous erythroderma ichthyosiformis congenita of Brocq. EHK is an hereditary skin disorder characterized by blistering and a marked thickening of the stratum corneum. At birth, affected individuals usually present with redness, blisters and superficial erosions due to cytolysis. Within a few weeks, the erythroderma and blister formation diminish and hyperkeratoses develop. Transmission is autosomal dominant, but most cases are sporadic. Defects in cytokeratin 10 are also a cause of annular epidermolytic ichthyosis (AEI), also known as cyclic ichthyosis with epidermolytic hyperkeratosis. AEI resembles clinical and histologic features of both epidermolytic hyperkeratosis and ichthyosis bullosa of Siemens.
Synonyms	Cytokeratin-10, CK10, CK-10, Keratin-10, KRT10, KPP, K10
Reference Data

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