Ptprc Mouse Monoclonal Antibody [Clone ID: OX-30]

Specifications

Product Data
Clone Name	OX-30
Applications	FC, IHC
Recommend Dilution	Flow cytometry (see protocol). Immunohistochemistry with frozen sections.
Reactivity	Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Lymph node glycoproteins and cells. Donor: BALB/c spleen Fusion Partner: NSO/U
Specificity	This monoclonal antibody recognizes a monomorphic determinant of the rat leukocyte common antigen.
Formulation	PBS and 0.02% NaN3 State: Purified State: Liquid purified Ig
Concentration	lot specific
Purification	Protein G Chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	protein tyrosine phosphatase, receptor type, C
Database Link	Entrez Gene 24699 Rat UniProt ID P04157
Background	The antigen recognized is a heavily glycosylated membrane glycoprotein of molecular weight 170,000 kDa on thymocytes but molecular weight 170,000-220,000 kDa on other leukocytes. The leukocyte common antigen (L-CA) is a major glycoprotein of haematopoietic cells but is not found on other tissues or erythroid cells. It is present on greater than 95% of thymocytes, bone marrow cells and thoracic duct lymphocytes. This molecule carries much of the carbohydrate of thymocytes and shows interesting heterogeneity amongst T lymphocytes and B lymphocytes
Synonyms	PTPRC, Leukocyte common antigen, L-CA, T200
Note	Protocol: FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-Rat cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50µl of this suspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add 0.5-1.0 µg* of this Ab. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody (FITC Goat anti-mouse IgG (H+L)) at 1:700 dilution. 9. Incubate the tubes at 4°C for 30-60 minutes. (It is recommended that the tubes are protected from light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C in media B. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 µl of 2M sodium azide in 100 mls). Results - Tissue Distribution: Rat Strain: Wistar Cell Concentration: 1x10e6 cells per test Antibody Concentration Used: 0.5 µg/10e6 cells Isotypic Control: Mouse IgG2a Cell Source Percentage of cells stained above control: Thymus: 99.9% Spleen: 97.4% Lymph Node: 90.6% Results - Strain Distribution: Antibody Concentration Used: 0.5 µg/10e6 cells Strains Tested: Wistar, Buffalo, Brown Norway, Fischer 344 Positive: Wistar, Buffalo, Brown Norway, Fischer 344 Negative: none
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.