CD44 Mouse Monoclonal Antibody [Clone ID: OX-49]

Specifications

Product Data
Clone Name	OX-49
Applications	FC, IHC, IP, WB
Recommend Dilution	This antibody is suitable for Immunoprecipitation, Flow cytometry (See protocol), Western Blotting and Immunohistochemisty on frozen and paraffin sections.
Reactivity	Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	T cell blasts.
Specificity	Reacts with rat CD44 (Pgp-1). This antibody recognizes an epitope on both standard CD44 and its splice variant.
Formulation	PBS buffer with 0.02% sodium azide as preservative. State: Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Protein G affinity chromatography.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Database Link	UniProt ID P26051
Background	This antigen is expressed on most leukocytes (except a sub population of B cells) and increases upon activation. This antibody binds extracellularly to the standard (S) form on rat leukocytes, but it is not known if they bind to the N-terminal region. It has also been reported that the antibody may bind to melanoma cell lines that express CD44V (splice variant form). CD44 is expressed on most leukocytes except a sub population of B cells. Its expression is increased on T and B blasts.
Synonyms	LHR, MDU2, MDU3, MIC4, CDw44, Epican, ECMR-III, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, PGP-1
Note	Protocol: FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Rat cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of this suspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test). 4. To each tube, add 1.0-0.5 µg* of CL110P or CL110PX. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody (FITC Goat anti-mouse IgG (H+L)) at 1:500 dilution. 9. Incubate the tubes at 4°C for 30-60 minutes. (It is recommended that the tubes are protected from light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C in media B. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 µl of 2M sodium azide in 100 mls). Results-Tissue Distribution: Rat Strain: Wistar Cell Concentration: 1 x 10e6 cells per tests Antibody Concentration Used: 0.2 µg/10e6 cells Isotypic Control: Mouse IgG2a. Cell Source-Percentage of cells stained above control: Thymus: 82.1% Spleen: 53.5% Lymph Node: 87.1% N.B. Appropriate control samples should always be included in any labelling studies. * For optimal results in various applications, it is recommended that each investigator determine dilutions appropriate for individual use.
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.