CD44 Mouse Monoclonal Antibody [Clone ID: OX-49]

Specifications

Product Data
Clone Name	OX-49
Applications	FC, IHC
Recommend Dilution	Flow cytometry (See protocol).  Immunohistochemistry on Frozen Sections and Paraffin Sections.
Reactivity	Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	T cell blasts.
Specificity	This anti-Rat CD44 monoclonal antibody recognizes an epitope on both standard CD44 and its splice variant.
Formulation	PBS with 0.02% sodium azide as preservative and EIA grade BSA as a stabilizer Label: FITC State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Protein G affinity chromatography
Conjugation	FITC
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Database Link	UniProt ID P26051
Background	This antigen is expressed on most leukocytes (except a sub population of B cells) and increases upon activation. This antibody binds extracellularly to the standard (S) form on rat leukocytes, but it is not known if they bind to the N-terminal region. It has also been reported that the antibody may bind to melanoma cell lines that express CD44V (splice variant form). CD44 is expressed on most leukocytes except a sub population of B cells. Its expression is increased on T and B blasts.
Synonyms	LHR, MDU2, MDU3, MIC4, CDw44, Epican, ECMR-III, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, PGP-1
Note	Protocol: FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Rat cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x107 cells/ml in media A. Add 50 µl of this suspension to each tube (each tube will then contain 1x106 cells, representing 1 test). 4. To each tube, add 1.0-0.5 µg* of CL110F or CL110FX. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes are protected from light since most fluorochromes are light sensitive). 7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 µl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 µl of 2M sodium azide in 100 mls). Results-Tissue Distribution: Rat Strain: Wistar Cell Concentration: 1 x 10e6 cells per tests Antibody Concentration Used: 1.0 µg/10e6 cells Isotypic Control: FITC Mouse IgG2a. Cell Source Percentage of cells stained above control: Thymus: 99.6% Spleen: 75.9% Lymph Node: 96.8% N.B. Appropriate control samples should always be included in any labelling studies. * For optimal results in various applications, it is recommended that each investigator determine dilutions appropriate for individual use.
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.