CNOT2 Mouse Monoclonal Antibody [Clone ID: OTI9B10]

Specifications

Product Data
Clone Name	OTI9B10
Applications	IHC, WB
Recommend Dilution	WB 1:2000, IHC 1:150
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human CNOT2(NP_055330) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	59.6 kDa
Gene Name	CCR4-NOT transcription complex subunit 2
Database Link	NP_055330 Entrez Gene 72068 MouseEntrez Gene 299805 RatEntrez Gene 4848 Human UniProt ID Q9NZN8
Background	This gene encodes a subunit of the multi-component CCR4-NOT complex. The CCR4-NOT complex regulates mRNA synthesis and degradation and is also thought to be involved in mRNA splicing, transport and localization. The encoded protein interacts with histone deacetylases and functions as a repressor of polymerase II transcription. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Dec 2010]
Synonyms	CDC36; HSPC131; NOT2; NOT2H
Reference Data
Protein Families	Transcription Factors
Protein Pathways	RNA degradation

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