PIP5K2 alpha (PIP4K2A) Mouse Monoclonal Antibody [Clone ID: OTI3D3]

Specifications

Product Data
Clone Name	OTI3D3
Applications	FC, IHC, WB
Recommend Dilution	WB: 1:200 - 1:1000, IHC 1:150, FLOW 1:100
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human PIP4K2A (NP_005019) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	46 kDa
Gene Name	phosphatidylinositol-5-phosphate 4-kinase type 2 alpha
Database Link	NP_005019 Entrez Gene 18718 MouseEntrez Gene 116723 RatEntrez Gene 5305 Human UniProt ID P48426
Background	Phosphatidylinositol-5,4-bisphosphate, the precursor to second messengers of the phosphoinositide signal transduction pathways, is thought to be involved in the regulation of secretion, cell proliferation, differentiation, and motility. The protein encoded by this gene is one of a family of enzymes capable of catalyzing the phosphorylation of phosphatidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring to form phosphatidylinositol-5,4-bisphosphate. The amino acid sequence of this enzyme does not show homology to other kinases, but the recombinant protein does exhibit kinase activity. This gene is a member of the phosphatidylinositol-5-phosphate 4-kinase family. [provided by RefSeq]
Synonyms	PI5P4KA; PIP5K2A; PIP5KII-alpha; PIP5KIIA; PIPK
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Inositol phosphate metabolism, Phosphatidylinositol signaling system, Regulation of actin cytoskeleton

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