TPMT Mouse Monoclonal Antibody [Clone ID: OTI2A2]

Specifications

Product Data
Clone Name	OTI2A2
Applications	IF, IHC, WB
Recommend Dilution	WB 1:1000, IHC 1:50, IF 1:100
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human TPMT (NP_000358) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	28.2 kDa
Gene Name	thiopurine S-methyltransferase
Database Link	NP_000358 Entrez Gene 7172 Human UniProt ID P51580
Background	This gene encodes the enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. Thiopurine drugs such as 6-mercaptopurine are used as chemotherapeutic agents. Genetic polymorphisms that affect this enzymatic activity are correlated with variations in sensitivity and toxicity to such drugs within individuals. A pseudogene for this locus is located on chromosome 18q.
Synonyms	OTTHUMP00000016076; S-adenosyl-L-methionine:thiopurine S-methyltransferase; thiopurine methyltransferase; thiopurine S-methyltransferase
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Drug metabolism - other enzymes

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