CYP7B1 Mouse Monoclonal Antibody [Clone ID: OTI7H10]

Specifications

Product Data
Clone Name	OTI7H10
Applications	WB
Recommend Dilution	WB 1:1000
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant protein expressed in E.coli corresponding to amino acids 350-506 of human Cyp7b1
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	58.1 kDa
Gene Name	cytochrome P450 family 7 subfamily B member 1
Database Link	NP_004811 Entrez Gene 9420 Human UniProt ID O75881
Background	Cyp7b1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway of extrahepatic tissues, which converts cholesterol to bile acids. This enzyme likely plays a minor role in total bile acid synthesis, but may also be involved in the development of atherosclerosis, neurosteroid metabolism and sex hormone synthesis
Synonyms	CBAS3; CP7B; SPG5A
Reference Data
Protein Families	Druggable Genome, P450, Transmembrane
Protein Pathways	Primary bile acid biosynthesis

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