Integrin alpha 6 (ITGA6) Rat Monoclonal Antibody [Clone ID: NKI-GoH3]

Specifications

Product Data
Clone Name	NKI-GoH3
Applications	FC, IF, IHC, WB
Recommend Dilution	Western Blot.  Flow Cytometry. Immunohistochemistry on Frozen Sections: 1/5-1/10. Positive Control: Epithelial tissues. Incubation time: 30 min at room temperature or overnight at 2-8°C.
Reactivity	Canine, Human, Monkey, Mouse, Porcine
Host	Rat
Clonality	Monoclonal
Immunogen	Mouse mammary tumor cells.
Specificity	In cutaneous melanocytic lesions, the level of expression of three integrin subunits (α2, α6, and β3) has been found to be correlated with tumor progression. This Monoclonal Antibody GoH3, directed against α6-integrins (Laminin receptor, VLA-6), shows a cytoplasmic staining pattern of uveal and cutaneous melanoma cells.  Antibody blocks binding of integrin α6 to Laminin P1 and E8. In Western Blot reactive with two major bands of 120 and 140 kDa under Non-Reducing Conditions.
Formulation	PBS, pH 7.4 State: Supernatant State: Cell Culture Supernatant Stabilizer: 0.5% BSA Preservative: 0.09% Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	integrin subunit alpha 6
Database Link	Entrez Gene 3655 Human UniProt ID P23229
Background	Integrins are important extracellular matrix (ECM) receptor proteins located on cell surfaces. They are hetrodimers composed of an alpha and a beta transmembrane glycoprotein subunit. Around twenty two different integrins (different alpha/ beta subunit combinations) are found in nature. Integrins are generally present in high concentrations at the cell surface, but, unlike most other cell surface receptors, they bind ligands with very low affinity. Due to their weak individual binding, integrins need to cluster and bind in groups in order to effectively bind the ECM. Integrins bind many different ligands including laminin. Each integrin is made up of a large N terminal extracellular domain that binds the ECM ligand and a small C terminal cytoplasmic domain that mediates interaction with the actin cytoskeleton and signaling function. Integrin alpha 6 complexes are receptors for laminins, which are components of basement membranes. Integrin alpha 6 complexes may play an important role in embryogenesis.
Synonyms	Integrin alpha-6, VLA-6, VLA6
Reference Data

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