pan Cytokeratin Mouse Monoclonal Antibody [Clone ID: Lu5]

Specifications

Product Data
Clone Name	Lu5
Applications	IHC
Recommend Dilution	Immunohistochemistry on Frozen Sections: 0.4-0.8 μg/ml (1/500-1/1000). Immunohistochemistry on Paraffin Sections: 2 μg/ml (1/200). Proteinase K pretreatment for antigen retrieval is recommended. Suggested Positive Control: Human tonsil.
Reactivity	Amphibian, Bovine, Chicken, Human, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human lung cancer cell line
Specificity	Monoclonal antibody BM4068 recognizes most subtypes of cytokeratins in cryofixed and paraffin sections and is ideally suited as a first order pan-epithelial marker: Clone Lu-5 stains an intracytoplasmic, formaldehyde (paraffin embedding) resistant epitope on the surface of cytokeratin filaments. The epitope recognized by this antibody is a formalin-resistant marker of great value in tumour diagnosis, located on the surface of cytokeratin filaments. It has been preserved during vertebrate evolution and can be shown in all species from amphibia to man. The epitope is present in most cytokeratin polypeptides of both the acidic (type I) and basic (type II) subfamily but does not occur in other cytoskeletal proteins. The epithelial specificity and the broad tissue and species cross-reactivity provide an excellent probe for the differential diagnosis of epithelial versus mesenchymal tumours, large cell lymphomas and neural tumours.
Formulation	PBS, pH 7.2 State: Aff - Purified State: Lyophilized purified Ig fraction Stabilizer: 5 mg/ml BSA Preservative: 0.05% Kathon
Reconstitution Method	Restore by adding 0.5 ml distilled water. This stock solution contains 0.4 mg/ml IgG.
Concentration	0.4 mg/ml (after reconstitution)
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Synonyms	pan Keratin, Cytokeratin pan-reactive
Note	Lu-5 has been tested on a wide variety of healthy and tumorous human tissues. 95% of epithelial tumours regardless of their localisation and grade of differentiation were detected (Von Overbeck et al. 1985). It is currently used for differentiating epithelial and mesothelial tumours from mesenchymal tumours, large cell lymphomas and neuronal tumours.
Reference Data

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