CD31 (PECAM1) Mouse Monoclonal Antibody [Clone ID: MEM-05]

Specifications

Product Data
Clone Name	MEM-05
Applications	FC, IHC
Recommend Dilution	Immunohistochemistry on Frozen Sections: 1-2 µg/ml (1/200-1/400). Immunohistochemistry on Paraffin Sections: 8 µg/ml (1/50). Proteinase K pretreatment for antigen retrieval is recommended. Suggested Positive Control: Human placenta. Has been described to work in FACS.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human CD31
Specificity	This antibody is specific for CD31.
Formulation	Stock solution contains PBS, pH 7.2 with 10 mg/ml BSA as a stabilizer and 0.09% Sodium Azide as a preservative. State: Lyophilized diluted ascites
Reconstitution Method	Restore by adding 0.5 ml distilled water to 0.4 mg/ml.
Conjugation	Unconjugated
Storage Condition	Store the lyophilized product at 2-8°C. Following reconstitution store in aliquots at -20°C. Avoid repeated freezing and thawing.
Gene Name	platelet and endothelial cell adhesion molecule 1
Database Link	Entrez Gene 5175 Human UniProt ID P16284
Background	CD31, also known as platelet endothelial cell adhesion molecule 1 (PECAM1), is a type I integral membrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is constitutively expressed on the surface of endothelial cells, and concentrated at the junction between them. It is also weakly expressed on many peripheral lymphoid cells and platelets. CD31 has been used to measure angiogenesis in association with tumor recurrence. Other studies have also indicated that CD31 and CD34 can be used as markers for myeloid progenitor cells and recognize different subsets of myeloid leukemia infiltrates (granular sarcomas). The CD31 antigen is found on cells lining and within the vasculature, and plays a role in the adhesive interaction between adjacent endothelial cells as well as between leukocytes and endothelial cells.
Synonyms	PECAM-1, EndoCAM, GPIIA'
Note	Protocol: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature 1. Wash in PBS 2. Block endogenous peroxidase 3. Wash in PBS 4. Block with 10 % normal goat serum in PBS for 30 min. in a humid chamber 5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber 6. Wash in PBS 7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1 h in a humid chamber 8. Wash in PBS 9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12 min. 10. Wash in PBS 11. Counterstain with Mayer's hemalum Protocol with formalin-fixed, paraffin-embedded sections: The whole procedure is performed at room temperature 1. Deparaffinize and rehydrate tissue section 2. Incubate the tissue section with proteinase K for 7 min. 3. Wash in distilled water 4. Block endogenous peroxidase 5. Wash in PBS 6. Block with 10 % normal goat serum in PBS for 30 min. in a humid chamber 7. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber 8. Wash in PBS 9. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1 h in a humid chamber 10. Wash in PBS 11. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12 min. 12. Wash in PBS 13. Counterstain with Mayer's hemalum
Reference Data

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