CD163 Mouse Monoclonal Antibody [Clone ID: ED2]

Specifications

Product Data
Clone Name	ED2
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	Western blot. Immunoprecipitation. Immunofluorescence.  Flow Cytometry: Use 10 µl of 1/10-1/100 diluted CD163 antibody to label 106 cells in 100 µl. Immunohistochemistry on Frozen Sections: 1/50-1/100.  Immunohistochemistry on Paraffin Sections: This product requires antigen retrieval using pronase prior to staining of paraffin sections. IHC on paraffin-embedded material following PLP fixation (Periodate-lysine-paraformaldehyde) - refer to Whiteland et al (Ref.5.) Histology Positive Control: Liver tissue.
Reactivity	Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Rat Spleen cell homogenate. Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0Ag 14 mouse myeloma cell line.
Specificity	This antibody recognises the Rat CD163 cell surface glycoprotein, a 175kD molecule also known as ED2.  It does not react with Human. Mouse anti Rat CD163, clone ED2 was shown to detect approximately 50% of peritoneal macrophages, a subset of splenic macrophages, and most tissue macrophages. However, no staining was observed in monocytes or alveolar macrophages (Dijkstra et al. 1985, Beelen et al. 1987). In freshly isolated bone marrow, expression of CD163 was limited to mature macrophages only (Barbe et al. 1990).
Formulation	PBS State: Purified State: Liquid purified IgG fraction from Tissue Culture Supernatant Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	CD163 molecule
Database Link	Entrez Gene 9332 Human UniProt ID Q86VB7
Background	CD163 is a scavenger receptor for the haemoglobin-haptoglobin complex, and is upregulated by glucocorticoids and IL-10. The extracellular portion of the receptor is regularly shed and can be found in the circulation. An important function of CD163 seems to be in the adhesion of monocytes to activated endothelial cells. CD163 is expressed by approximately 50% of peritoneal macrophages, a subset of splenic macrophages, and by macrophages in most other tissues. However, it is not expressed by monocytes, alveolar macrophages or microglial cells.
Synonyms	M130, Hemoglobin scavenger receptor, Macrophage marker
Reference Data
Protein Families	Druggable Genome, Secreted Protein, Transmembrane

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