PLGF (PGF) (N-term) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Western blot: At 1-2μg/ml with the appropriate system to detect PLGF in cells and tissues. Immunohistochemistry on paraffin sections: At 1-2μg/ml to detect PLGF in formalin fixed and paraffin embedded tissues. Immunocytochemistry.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide corresponding to a sequence mapping near the N-terminal of human PLGF
Specificity	This antibody detects Placenta growth factor / PGF (N-term). No cross reactivity with other proteins.
Formulation	50% glycerol, 0.9mg NaCl, 0.2mg Na2HPO4 State: Aff - Purified State: Lyophilized Ig fraction
Reconstitution Method	1.2% sodium acetate or neutral PBS. If 0.5ml of PBS is used, the antibody concentration will be 100μg/ml.
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Gene Name	placental growth factor
Database Link	Entrez Gene 18654 MouseEntrez Gene 94203 RatEntrez Gene 5228 Human UniProt ID P49763
Background	Placental growth factor (PGF, also known as PLGF) codes for an angiogenic factor expressed in placental tissue that is similar to vascular permeability factor/vascular endothelial growth factor (VPF/VEGF). It is a glycosylated dimeric secreted protein able to stimulate endothelial cell growth in vitro. PGF is located on chromosome 14 and has been conserved in evolution. It belongs to the family of vascular endothelial growth factors (VEGFs) and binds to the flt-1 VEGF receptor. PLGF-2, which is a PLGF isoform, binds neuropilin-1 and 2 in a heparin-dependent manner. PGF regulates inter-and intra molecular cross talk between the VEGF RTKs Flt1 and Flk1 and stimulates the phosphorylation of specific Flt1 tyrosine residues and the expression of distinct downstream target genes. Moreover, it plays an important role in pathological angiogenic events and with exerting its biological activities through binding to VEGFR1.
Synonyms	PGFL, PLGF, PlGF
Reference Data
Protein Families	Druggable Genome, Secreted Protein
Protein Pathways	Bladder cancer, Focal adhesion, mTOR signaling pathway, Pancreatic cancer, Pathways in cancer, Renal cell carcinoma

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