Cytochrome P450 2E1 (CYP2E1) (C-term) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Western blot: At 1-2μg/ml with the appropriate system to detect P450ⅡE1 in cells and tissues. Immunohistochemistry on paraffin sections: At 1-2μg/ml to detect P450ⅡE1 in formalin fixed and paraffin embedded tissues. Boiling the sections is required. Immunohistochemistry on frozen sections: At 1-2μg/ml to detect P450ⅡE1 in formalin or acetone fixed tissues.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide mapping at the C-terminal of human P450ⅡE1
Specificity	This antibody detects CYP2E1 (C-term).
Formulation	5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3 State: Aff - Purified State: Lyophilized Ig fraction
Reconstitution Method	0.2ml of distilled water will yield a concentration of 500μg/ml.
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Gene Name	cytochrome P450 family 2 subfamily E member 1
Database Link	Entrez Gene 13106 MouseEntrez Gene 25086 RatEntrez Gene 1571 Human UniProt ID P05181
Background	P450IIE1, also known as CYP2E1, is a member of the P450IIE subfamily which is ethanol-inducible. It has at least 1 gene which is mapped to 10q24.3-qter, and a second is likely in rat and in man1. Both the rat and human proteins encoded by this gene contain 493 amino acids and calculated molecular masses of 56,634 and 56,916 daltons, respectively2. In addition, genetic polymorphisms in the 5-prime flanking region of the human P450IIE1 gene affected its binding of transacting factor and changed its transcriptional regulation, which may lead to interindividual differences of microsomal drug oxidation activity3. P450IIE1 is an important enzyme for the catalysis of the conversion of ethanol to acetaldehyde and to acetate in humans,and it is also involved in the metabolism of nitrosamines. Due to the possible correlation of P450IIE1 genes with malignancy, clinical studies of RFLP patterns of these genes in cancer may be useful.
Synonyms	Cytochrome P450 2E1, CYP2E, CYPIIE1, Cytochrome P450-J
Reference Data
Protein Families	Druggable Genome, P450, Transmembrane
Protein Pathways	Arachidonic acid metabolism, Drug metabolism - cytochrome P450, Linoleic acid metabolism, Metabolic pathways, Metabolism of xenobiotics by cytochrome P450

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