DKK2 (C-term) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	ELISA 1:1,000. Western blot 1:100 - 1:500. Immunohistochemistry 1:50 - 1:100.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	This polyclonal antibody was raised against a peptide corresponding to a sequence selected for the C-terminal region of human DKK2.
Specificity	This antibody detects DKK2 at C-term.
Formulation	PBS with 0.09% (W/V) sodium azide State: Purified State: Liquid Ig fraction
Concentration	lot specific
Purification	Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Conjugation	Unconjugated
Storage Condition	Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Gene Name	dickkopf WNT signaling pathway inhibitor 2
Database Link	Entrez Gene 27123 Human UniProt ID Q9UBU2
Background	The 259-amino acid DKK2 protein, like DKK1, DKK3, and DKK4, possesses an N-terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cys residues each. The second cys region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis revealed expression of 4.0- and 4.5-kb DKK2 transcripts in heart, brain, skeletal muscle, and lung. Western blot analysis showed that DKK2 is secreted as a 15- to 17-kD protein. Functional analysis determined that DKK2 does not block Xenopus Wnt8 induction of a secondary axis in frog embryos.
Synonyms	Dickkopf-related protein 2, Dkk-2
Note	Molecular weight: 28447 Da
Reference Data

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