Estrogen Receptor 1 (ESR1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Western blot: 1/500-1/1000. Immunohistochemistry on paraffin sections: 1/50-1/200. Immunofluorescence: 1/50-1/200.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide, corresponding to amino acids 501-550 of Human ERα.
Specificity	This antibody detects endogenous levels of ER-alpha protein. (region surrounding Lys531)
Formulation	Phosphate buffered saline (PBS), pH 7.2. State: Aff - Purified State: Liquid purified Ig fraction Preservative: 0.05% sodium azide
Concentration	1.0 mg/ml
Purification	Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	~ 66 kDa
Gene Name	estrogen receptor 1
Database Link	Entrez Gene 2099 Human UniProt ID P03372
Background	Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor uperfamily of ligand-activated transcription factors. Estrogen receptors, ncluding ERalpha and ERbeta, contain DNA binding and ligand binding domains nd are critically involved in regulating the normal function of reproductive issues. ERalpha and ERbeta have been shown to be differentially activated by various igands. Receptor-ligand interactions trigger a cascade of events, including issociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory lements in target genes. Evidence suggests that ERalpha and ERbeta may be egulated by distinct mechanisms even though they share many functional characteristics.
Synonyms	ER alpha, Estradiol receptor, ESR1, ESR, NR3A1
Reference Data

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