Vimentin (VIM) Mouse Monoclonal Antibody [Clone ID: SPM576]

CAT#: AM50186PU-S

Vimentin (VIM) mouse monoclonal antibody, clone SPM576, Purified

Size: 20 ug 100 ug



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CNY 5,606.00


货期*
5周

规格
    • 100 ug

Product images

Specifications

Product Data
Clone Name SPM576
Applications FC, IF, IHC, IP, WB
Recommend Dilution ELISA: Use BSA free Antibody for coating.
Flow Cytometry: 0.5-1 µg/million cells 
Immunofluorescence: 1-2 µg/ml. 
Western Blotting: 0.5-1 µg/ml. 
Immunoprecipitation: 1-2 µg/500 µg protein lysate. 
Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Positive Control: Jurkat cells, Sarcomas, Melanomas.
Reactivity Bovine, Canine, Chicken, Feline, Goat, Human, Porcine
Host Mouse
Clonality Monoclonal
Immunogen Human vimentin recombinant protein.
Specificity This MAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP’s) such as Desmin, Keratin, neurofilament, and glial fibrillary acid protein. Anti-Vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.
Cellular Localization: Cytoplasmic. 
Negative Species: Mouse and Rat.
Formulation 10mM PBS
State: Purified
State: Liquid purified IgG fraction from Bioreactor Concentrate
Stabilizer: 0.05% BSA
Preservative: 0.05% Sodium Azide
Concentration lot specific
Purification Protein A/G Chromatography
Conjugation Unconjugated
Storage Condition Store undiluted at 2-8°C.
Predicted Protein Size 57-60 kDa
Gene Name vimentin
Synonyms VIM
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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