CD63 (Late Endosomes Marker) Mouse Monoclonal Antibody [Clone ID: SPM524]

Specifications

Product Data
Clone Name	SPM524
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	ELISA: Use BSA Free antibody for coating. Flow Cytometry: 0.5-1 µg/106 cells.  Immunofluorescence: 1-2 µg/ml.  Western Blotting: 0.5-1 µg/ml.  Immunoprecipitation: 1-2 µg/500 µg protein lysate.  Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.  Recommended Positive Control: SK-MEL-28, HL60, THP-1 or NIH/3T3 cells. Melanoma or lymphoma.
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Smooth plasma membrane fraction of MeWo cells.
Specificity	This Monoclonal antibody recognizes Human and Mouse CD63. Other species not tested. Cellular Localization: Cytoplamsic.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Predicted Protein Size	26 kDa (core protein); 30-60 kDa (glycosylated)
Gene Name	CD63 molecule
Database Link	Entrez Gene 967 Human UniProt ID P08962
Background	The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.
Synonyms	OMA81H, Granulophysin, Tetraspanin-30, MLA1, TSPAN30, ME491
Reference Data

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