Cd5 Rat Monoclonal Antibody [Clone ID: 53-7.3]

Specifications

Product Data
Clone Name	53-7.3
Applications	FC, IHC, IP
Recommend Dilution	This clone (53-7.3) has been reported to work in Immunohistochemistry on Acetone Fixed Frozen Sections and Zinc Fixed Paraffin Sections, Immunoprecipitation and Flow Cytometry.
Reactivity	Human, Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Mouse thymus or spleen from Balb/c mouse thymus or spleen.
Specificity	This CD5 (Ly-1) Monoclonal Antibody is specific for the Mouse / Human CD5 antigen. Test Results for tissue distribution by flow cytometry: Cell concentration = 1x106 cells per test Antibody concentration = 0.2 µg/106 cells Percentage of cells stained above control: 95.9% - Thymus 39.19% -Spleen 3.66% - Bone Marrow
Formulation	PBS containing 0.02% Sodium Azide as preservative State: Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Protein G Chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in alqiuots) -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	CD5 antigen
Database Link	Entrez Gene 921 HumanEntrez Gene 12507 Mouse UniProt ID P13379
Background	CD5 is a 55kDa T lymphocyte single chain transmembrane glycoprotein. It is present on all mature T lymphocytes, on most thymocytes and on many T cell leukemias and lymphomas. It reacts with a subpopulation of activated B cells. CD5/Lyt1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells. It has been identified as the major ligand of the B cell antigen CD72. The frequency of CD5+ B cells exhibits strain dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B1 cells. Binding of CD5 on the T cell surface can augment alloantigen or mitogen induced lymphocyte proliferation and induces increased cytosolic free calcium, IL2 secretion, and IL2R expression. It has been proposed that CD5 negatively regulates signal transduction mediated by the T cell and B cell receptors.
Synonyms	CD5, LEU1
Reference Data

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