ATG5 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 7C6]

Specifications

Product Data
Clone Name	7C6
Applications	FC, IF, WB
Recommend Dilution	Flow Cytometry. Immunobotting (Western Blot): 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking buffer and blot incubation buffer. Immunocytochemistry: 0.5 µg/ml. Included Postitive Control: Cell lysate from untreated SH-SY5Y cells (See Protocols).
Reactivity	Canine, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant ATG5
Specificity	Recognizes the ATG5-ATG12 protein complex at 55 kDa and ATG5 at 33 kDa in Immunoblot application.
Formulation	PBS State: Purified State: Lyophilized purified IgG fraction Stabilizer: PEG and Sucrose Preservative: 0.09% Sodium Azide
Reconstitution Method	Restore with 1ml H2O (15 min, RT).
Purification	Purified from Serum-Free Cell Culture Supernatant by subsequent ultrafiltration and Size Exclusion Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -20°C to -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 2-8°C up to 3 months.
Predicted Protein Size	24, 33, 55 kDa
Gene Name	autophagy related 5
Database Link	Entrez Gene 11793 MouseEntrez Gene 365601 RatEntrez Gene 9474 Human UniProt ID Q9H1Y0
Background	Autophagy as a response to cellular stress and starvation is an alternative process of proteasomal degradation for some long-lived proteins and organelles. Alterations in the autophagic-lysosomal compartment have been linked to neuronal death in many neurodegenerative disorders as well as in transmissible neuronal pathologies (prion diseases). The gene product of autophagy-related gene 5 (ATG5) is required for autophagosome-formation. ATG5 also enhances the susceptibility towards apoptotic stimuli. Like Bcl-2, ATG5 exhibits a dual function by modulating both autophagy and apoptosis.
Synonyms	ASP, APG5-like, Autophagy protein 5
Note	Protocol: Positive Control: Cell lysate from untreated SH-SY5Y cells. Formulation: Lyophilized cell lysate from Serum starved SH-SY5Y cells. Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The Positive Control lysate is recommended for Immunoblot applications. 20µl of Positive Control correspond to ca. 20.000 cells. Use 20µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Shelf life: one year from despatch. The Lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as Immunoprecipitation.
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Regulation of autophagy, RIG-I-like receptor signaling pathway

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