STAT1 pSer727 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 12C5]

Specifications

Product Data
Clone Name	12C5
Applications	ELISA, WB
Recommend Dilution	Western Blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. ELISA: 0.05 µg/ml.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic phosphopeptide conjuagted to KLH
Specificity	This antibody specifically recognizes STAT1 phosphorylated at Ser 727. The antibody does not crossreact with the non-phosphorylated form of STAT1 nor with unrelated serine-phosphorylated proteins.
Formulation	0.1mg/ml in in PBS/0.09% Na-Azide/PEG and Sucrose/50% Glycerol State: Purified State: Liquid purified IgG
Purification	Size exclusion chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody (in alliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	signal transducer and activator of transcription 1
Database Link	Entrez Gene 6772 Human UniProt ID P42224
Background	The STAT proteins serve as both cytoplasmic signal transducers and nuclear activators of transcription. STATs are mediators involved in cytokine signalling. In response to a specific cytokine signal, STAT proteins are phosphorylated on conserved tyrosine residues. Phosphorylated STAT proteins dimerize via their SH2 domains and move to the nucleus. The STAT dimers bind to specific DNA elements resulting in transcriptional regulation of downstream target genes. STAT1 is activated by phosphorylation at serine 727. The phosphorylation state of Ser 727 regulates transcription and apoptosis. STAT1 can bind to DNA as heterodimer with STAT3.
Synonyms	DKFZp686B04100; ISGF-3; OTTHUMP00000205845; STAT91
Reference Data

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