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CD47 (Locus ID 961) Human shRNA

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Specifications Related Products Product Manual FAQs
SKU Format Description Vector Price Availability*  
TR305509 Retroviral plasmids
  • CD47 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector (Gene ID = 961). 5µg purified plasmid DNA per construct
  • Non-effective 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.



¥6790 10-14工作日

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* These shRNA constructs were designed to be effective against maximum number of transcriptional variants at this gene locus.

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Reference Data
RefSeq: NM_001777NM_198793NM_001025079NM_001025080XM_005247908XR_241522XR_241521
XR_924218XR_924219XR_001740374XR_001740375XR_924220XM_005247909XM_017007536
Synonyms: IAP, OA3, MER6
Summary: This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2010].
Performance Guranteed:
OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.

For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

 

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