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OriGene proteins in recent publications

Fully functional proteins purified from transient TrueORF cDNA clone transfections.  Researchers purified GEN1 protein using anti-FLAG M2 affinity column and determined it resolved Holliday junction X0 efficiently in vitro, thus confirming GEN1 is human Holliday junction resolvase.

Identification of Holliday junction resolvases from humans and yeast, Stephen C. Y. Ip, Ulrich Rass, Miguel G. Blanco, Helen R. Flynn, J. Mark Skehel, Stephen C. West, Nature 456, 357 - 361 (20 Nov 2008), doi: 10.1038/nature07470,


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DDX4 (NM_024415) Purified Human Protein

Specifications Citations Customer Service Product Documents
Cat. No. Description Datasheet Price Availability*  
TP310628 Recombinant protein of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 (DDX4), transcript variant 1, 20 ug (Larger pack size?) Product Datasheet ¥6650.00
¥2900.00
3-4周
TA50011-100 4C5, Anti-DDK monoclonal antibody (100 μL) ¥1000 现货
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Also for DDX4 (NM_024415)

OriGene Data
Species:Human Expression Host:HEK293 cells
Expression cDNA Clone or AA Sequence
Recombinant protein was produced with TrueORF clone, RC210628. Click on the TrueORF clone link to view cDNA and protein sequences.
Tag:C-terminal MYC/DDK Predicted MW:79.1 kDa
Purity:> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration:>50 ug/mL as determined by microplate BCA method
Buffer:25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Protein Families:
Protein Pathways:
Protein Data
  Protein Image
 

Reference Data
RefSeq: NP_077726 RefSeq Size: 2471 RefSeq ORF: 2175
LocusID: 54514 Cytogenetic: 5q11.2  
Synonyms : VASA; VASA
Summary: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009].
*: Inventory for certain proteins may be limited due to low expression level. Delivery time may vary from web posted schedule. Contact techsupport@origene.com for specific inventory information
**: DDK-tag is the same as FLAG tag. Flag? is a registered trademark of Sigma-Aldrich

 

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