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OriGene联合CELL杂志共同出版的
CRISPR论文集
Video overview of cDNA clone
offering
Video brochure for cDNA clone
OriGene cDNA clones in recent publications
Epilepsy-associated GRIN2A mutations reduce NMDA receptor trafficking and agonist potency - molecular profiling and functional rescue Sci Rep 2017 [GRIN2A]

Matrix metalloproteinase processing of PTHrP yields a selective regulator of osteogenesis, PTHrP1-17 Oncogene 2017 [PTH1R]

Whole-Exome Sequencing of Congenital Glaucoma Patients Reveals Hypermorphic Variants in GPATCH3, a New Gene Involved in Ocular and Craniofacial Development. Sci Rep 2017 [GPATCH3]

Ziploc-ing the Structure 2.0: endoplasmic reticulum resident peptidyl prolyl isomerases show different activities toward hydroxyproline J. Biol. Chem. 2017 [FKBP2]

Transfection Reagents

Researchers need to do gene overexpression (cDNA clones), gene knockdown (siRNA or shRNA) andgene knockout (CRISPR) to study gene functions. Effectively deliver those gene related products into cellsis critical for their research. OriGene provides a broad range of transfection reagents for all of thesedifferent gene formats.

Selection Guide

Applications Transfection Reagents Features & Cell Types
Plasmid DNATurbofectin 8.0™
  • Superior performance, comparable with FuGeneR 6
  • Broad spectrum for most Commonly used established cell lines, such as Hela, HEK293, MCF, 3T3, COS7, A549, etc.
Viromer RED,Viromer PLASMID
  • Virus-like polymer
  • Active endosome escape leading to high transfection efficiency
  • Best for hard-to-transfect and primary cells, such as human primary macrophages, primary cardiomyocytes, MEF, THP1, HACAT etc. Click to see customer's data
MegaTran   New
  • Economical choice
  • Excellent transfection efficiency
  • Broad cell spectrum (MegaTran 2.0)
293Tran   New
  • Formulated specially for HEK293 transfection
  • High transfection efficiency
  • Low cytotoxicity
siRNAsiTran   New
  • High efficiency and low cytotoxicity
  • Commonly used cell lines and primary cells, such as human primary T cells
Viromer BLUE, GREEN
  • Virus-like polymer
  • Active endosome escape leading to high transfection efficiency
  • Best for hard-to-transfect and primary cells, such as CACO-2, Kasumi-4, SH-SY5Y cell lines and human primary macrophages, monocytes and neurons, etc. Click to see customer's data
mRNAViromer RED , ViromermRNA
  • Virus-like polymer
  • Active endosome escape leading to high transfection efficiency
  • Tested in difficult-to-transfect and primary cells, such as human primary monocytes and dendritic cells
CRISPR RNP Viromer CRISPR
  • Efficient CRISPR RNP (gRNA and Cas9 protein complex) delivery
  • Superior transfection for genome editing
  • Higher reliability and efficiency of final editing compared to lipofection orelectroporation
Lentivirus ProductionLentiTran    New
  • Exceptional high titer lentivirus production
  • Simple & robust transfection procedure
  • Very affordable

  • TurboFectin 8.0™ - Lipid/histone based transfection reagent
    是针对核酸转染真核细胞所优化的一种高效率的新型转染试剂,由专利配方的脂质体和组蛋白混合物,溶在80%的酒精中。适用于在各种不同构建类型的细胞中转染TrueClone®/TrueORF®克隆 (基因过表达) 以及 HuSH-29® (用于基因沉默的shRNA)。

    • 性能优越: 与FuGENE®6 相媲美的高转染效率和低毒性
    • 应用广泛: 使用TurboFectin 8.0 已经成功转染超过100种细胞系及原代细胞类型
    • 价格优惠: 比FuGENE®价格便宜

  • MegaTran - 非脂质体聚合物的转染试剂
    MegaTran 2.0是一种非脂质体聚合物的转染试剂,专为大量细胞DNA转染和大规模蛋白质生产而设计和制造。

    • 高效的转染效率和低毒性
    • 通过瞬时转染提高蛋白质表达量
    • 与其他常用的转染试剂相比具有高性价比

  • siTran – 用于转染siRNA双链体
    siTran是一种全新的转染试剂,专门用于瞬时转染,高通量筛选等siRNA双链体.siTran 1.0在转染质粒DNA方面也非常有效。

    • 用于siRNA双链体和质粒DNA的双重用途试剂
    • 效率高,细胞毒性低
    • 已成功用于转染T细胞

  • Viromer – Virus-like polymer –病毒样多聚物-高效转染试剂 Viromer-病毒样聚合物-高效内体逃逸的转染试剂
    Viromer作为一种新的突破性技术,可利用病毒的融合机制(由此得名)包装成多聚化合物来介导质粒或siRNA转染。

    • 超高的转染效率、高效的内体逃逸、能够最大化转染效率并降低背景。
    • 高效、低毒
    • 非脂质体,因此不会参与细胞的代谢,特别是脂质的代谢。
    • 可用于反向转染,适用于高通量筛选

  • 293Tran – 专门用于转染HEK293细胞
    293Tran专为HEK293细胞配制,具有高转染效率。293Tran是一种非脂质体聚合物和可生物降解的转染试剂。

    • 专为HEK293 -最大化HEK293细胞的转染性能
    • 高转染效率 - 实现75-85%的转染效率
    • 低细胞毒性 - 维持细胞密度并减少实验偏差

  • LentiTran – 专为高滴度慢病毒生产而配制
    LentiTran是一种非脂质体聚合物的转染试剂,用于HEK293T细胞中的高滴度慢病毒生产。

    • 特别适合高滴度的慢病毒生产
    • 简单而有效的转染程序
    • 高性价比

 

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